, Volume 34, Issue 10, pp 1265–1266 | Cite as

Purification of acidic Z protein from human liver

  • K. Kamisaka
  • M. Hirano
  • M. Tsuru


Preparation of Z protein from human liver is described. Z protein consists of 2 forms which have different isoelectric points, pI 5.8 and pI 8.7, respectively. The acidic Z protein has a molecular weight of about 11,000 and has binding affinity for BSP using gel filtration.


Molecular Weight Purification Filtration Binding Affinity Human Liver 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    A.J. Levi, Z. Gatmaitan and I.M. Arias, J. clin. Invest.48, 2156 (1969).PubMedGoogle Scholar
  2. 2.
    H. Reye, A.J. Levi and I.M. Arias, J. clin. Invest.50, 2243 (1971).Google Scholar
  3. 3.
    G. Litwack, B. Kettere and I.M. Arias, Nature234, 466 (1971).PubMedGoogle Scholar
  4. 4.
    K. Kamisaka, I. Listowsky, Z. Gatmaintan and I.M. Arias, Biochemistry14, 2175 (1975).CrossRefPubMedGoogle Scholar
  5. 5.
    B. Ketterer, P. Ross-Mansell and J.K. Whitehead, Biochem. J.103, 316 (1967).PubMedGoogle Scholar
  6. 6.
    S. Mishkin, L. Stein, Z. Gatmaitan and I.M. Arias, Biochem. biophys. Res. Commun.47, 997 (1972).CrossRefPubMedGoogle Scholar
  7. 7.
    S. Mishkin and R. Turcotte, Biochem. biophys. Res. Commun.60, 376 (1974).CrossRefPubMedGoogle Scholar
  8. 8.
    R.K. Ockner, J.A. Manning, R.B. Poppenhausen and W.K.L. Ho, Science177, 56 (1972).PubMedGoogle Scholar
  9. 9.
    R.K. Ockner and J.A. Manning, J. clin. Invest.54, 326 (1974).PubMedGoogle Scholar
  10. 10.
    B. Ketteter, E. Tipping, D. Beale and J.A.T.P. Meuwissen, Trans. Biochem. Soc.3, 626 (1975).Google Scholar
  11. 11.
    B. Ketterer, E. Tipping and J.F. Hackney, Biochem. J.155, 511 (1976).PubMedGoogle Scholar
  12. 12.
    M. Warner and A.H. Neim. Can. J. Physiol. Pharmac.54, 493 (1975).Google Scholar
  13. 13.
    K. Kamisaka, I. Listowsky, Z. Gatmaitan and I.M. Arias, Biochim. biophys. Acta393, 24 (1975).PubMedGoogle Scholar
  14. 14.
    K. Weber and M. Osborn, J. biol. Chem.244, 4406 (1969).PubMedGoogle Scholar
  15. 15.
    C.W. Wringley, Meth. Enzym.22, 559 (1972).Google Scholar
  16. 16.
    N. Malik and A. Berrie, Analyt. Biochem.22, 559 (1972).Google Scholar

Copyright information

© Birkhäuser Verlag 1978

Authors and Affiliations

  • K. Kamisaka
    • 1
  • M. Hirano
    • 1
  • M. Tsuru
    • 1
  1. 1.The Second Department of Internal MedicineUniversity of TokyoTokyo(Japan)

Personalised recommendations