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, Volume 11, Issue 6–7, pp 646–648 | Cite as

Role of leucocyte procoagulant activity in endotoxin-induced DIC: Evidence from comparative studies in rats and rabbits

  • Nicola Semeraro
  • Mario Colucci
  • Luciana Mussoni
  • Maria B. Donati
Proceedings of the 3rd European Workshop on Inflammation, Paris, 2–3 March 1981 Inflammation Revisited: General Topics

Abstract

We have investigated the ability of rat and rabbit leucocytes to generate coagulant activity (PCA) in response to endotoxinin vitro andin vivo. On prolonged incubation with endotoxin (10 μg/ml f.c.) isolated rabbit leucocytes developed strong PCA as measured by clotting and amidolytic assay. In constrast, rat leucocytes failed to produce any PCA even in the presence of huge amounts of endotoxin (200 μg/ml f.c.). When rabbits were given two spaced endotoxin injections (25 μg/kg b.w., 24 h apart) blood leucocytes harvested 30–60 min after the second injection consistently showed marked PCA. Again, unlike the rabbit, rat leucocytes obtained after two endotoxin injections (up 2 mg/kg b.w.) were completely devoid of PCA. These findings support the view that leucocytes are involved in endotoxin-induced disseminated intravascular coagulation in rabbits. On the other hand the poor response of rat leucocytes to endotoxin might help explain the resistance of rats to DIC and Sanarelli-Shwartzman reaction.

Keywords

Huge Amount Poor Response Disseminate Intravascular Coagulation Prolonged Incubation Procoagulant Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1981

Authors and Affiliations

  • Nicola Semeraro
    • 1
    • 2
  • Mario Colucci
    • 1
    • 2
  • Luciana Mussoni
    • 1
    • 2
  • Maria B. Donati
    • 1
    • 2
  1. 1.Istituto di Ricerche Farmacologiche ‘Mario Negri’Milan
  2. 2.Istituto di Microbiologia, UniversitàBariItaly

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