Agents and Actions

, Volume 11, Issue 6–7, pp 536–537 | Cite as

The importance of the production of phosphatidic acid for the release of arachidonic acid in stimulated platelets

  • Eduardo G. Lapetina
  • M. Motasim Billah
Proceedings of the 3rd European Workshop on Inflammation, Paris, 2–3 March 1981 Phospholipase: Role in Cellular Activation

Abstract

Phosphatidic acid is formed by platelets as a result of the combined activities of phospholipase C and diacylglycerol kinase. The initial thrombin-activated phospholipase C-mediated reaction is quinacrine insensitive and is followed by quinacrine-sensitive phospholipase A2 activities. The phosphatidic acid released probably results in cellular calcium gating, which among other actions, might release arachidonic acid by activating phospholipases A2: Indeed, we have detected a phosphatidic acid-specific phospholipase A2 in platelets, which might have an important role in the liberation of arachidonic acid.

Keywords

Calcium Arachidonic Acid Combine Activity Phosphatidic Acid Quinacrine 

References

  1. [1]
    E.G. Lapetina andP. Cuatrecasas, Biochim. Biophys. Acta573, 394–402 (1979).PubMedGoogle Scholar
  2. [2]
    M.J. Broekman, M.W. Ward andA.J. Marcus, J. Clin. Invest.66, 275–283 (1980).PubMedGoogle Scholar
  3. [3]
    R. Walenga, J.Y. Vanderhoek andM.B. Feinstein, J. Biol. Chem.255, 6024–6027 (1980).PubMedGoogle Scholar
  4. [4]
    E.G. Lapetina, M.M. Billah andP. Cuatrecasas, J. Biol. Chem.256, 5037–5040 (1981).PubMedGoogle Scholar
  5. [5]
    E.G. Lapetina, M.M. Billah andP. Cuatrecasas, Nature, New Biology292, 367–369 (1981).Google Scholar
  6. [6]
    R.L. Bell, D.A. Kennerly, N. Stanford andP.W. Majerus, Proc. Natn. Acad. Sci. USA76, 3238–3241 (1979).Google Scholar
  7. [7]
    M.M. Billah, E.G. Lapetina andP. Cuatrecasas, J. Biol. Chem.256, 5399–5403 (1981).PubMedGoogle Scholar
  8. [8]
    M.M. Billah, E.G. Lapetina andP. Cuatrecasas, J. Biol. Chem.255, 10227–10231 (1980).PubMedGoogle Scholar
  9. [9]
    R.F. Irvine andR.M.C. Dawson, Biochem. Biophys. Res. Comm.91, 1399–1405 (1979).CrossRefPubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1981

Authors and Affiliations

  • Eduardo G. Lapetina
    • 1
  • M. Motasim Billah
    • 1
  1. 1.Department of Molecular BiologyThe Wellcome Research LaboratoriesResearch Triangle ParkUSA

Personalised recommendations