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The localization of zinc-65 in germinating corn tissues

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Summary

The distribution of zinc in the different solubility groups of proteins and cell wall constituents was studied, using Zn-65 in small maize plants.

Most of the Zn-65 was recovered in the soluble ‘protein’ fraction. The highest values correspond to the solubility groups for basic proteins, albumines and globulines. The prolamines and glutelines also contained an appreciable proportion. Non-dialyzable zinc percentages varied considerably between 0.1 per cent and 19.0 per cent, of the zinc absorbed by a tissue being the lowest figures characteristic for the saline extracts and especially for the grains.

Between the cell wall constituents, most Zn-65 was present in the solubility groups of protopectines and hemicelluloses. After different extractions, a rather tightly bound zinc fraction remains attached to the cell wall. This can only be separated by boiling with KOH, leaving zinc-free cell wall material. The pure α-cellulose walls contained no Zn-65.

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Literature cited

  1. Bonner, J. S., Plant Biochemistry. Academic Press, N. Y. 537 pp. (1950).

  2. Bonner, J. S., The Hormonal Control of Plant Growth. Harvey Lectures, Ser.48, 1–34 (1954).

    Google Scholar 

  3. Boroughs, H. and Bonner, J., Effects of indoleacetic acid on metabolic pathways. Arch. Biochem. Biophys.46, 279–290 (1953).

    Article  PubMed  Google Scholar 

  4. Bukovac, M. F. and Wittwer, S. H., Absorption and mobility of foliar applied nutrients. Plant Phys.32, 428–435 (1957).

    Google Scholar 

  5. Davidson, J. N., The Biochemistry of Nucleic Acids. 4th. ed. J. Wiley, N. Y. 288 pp. (1957).

  6. Friden, C., The dissociation of glutamic dehydrogenase by reduced diphosphopyridine nucleotide (DNPH). Biochim. et Biophys. Acta27, 431–32 (1958).

    Article  Google Scholar 

  7. Galston, A. W. and Kaur, R., An effect of auxines on the heat coagulability of the proteins of growing plant cells. Proc. Natl. Acad. Sci. U.S.45, 1587–1590 (1959).

    Google Scholar 

  8. Galston, A. W. and Purves, W. K., The mechanism of action of auxin. Ann. Rev. Plant Physiol11, 239–276 (1960).

    Article  Google Scholar 

  9. Hawk, P. B., Oser, B. L., and Summerson, W. H., Practical Physiological Chemistry. 13th. ed. McGraw-Hill, N. Y. 1439 pp. (1954).

  10. Hewitt, E. J., Bolle-Jones, E. W., and Miles, P., The production of copper, zinc, and molibdenum deficiencies in crop plants grown in sand culture with special reference to some effects of water supply and seed reserves. Plant and Soil5, 205–222 (1954).

    Article  Google Scholar 

  11. Hewitt, W. and Gardner, M. E., Some studies on the adsorption of zinc sulfate in Thomson seedless grape canes. Plant Physiol31, 393–99 (1956).

    Google Scholar 

  12. Hoch, F. L. and Vallee, B. L., The metabolic role of zinc.In Trace Elements. eds. C. A. Lamb, O. G. Bentley, and J. M. Beattie. pp. 337–363. Academic Press Inc., N. Y. (1948).

    Google Scholar 

  13. Kägli, J. H. R. and Vallee, B. L., The role of zinc in the alcohol dehydrogenase. V. The effect of metal binding agents on the structure of yeast alcohol dehydrogenase molecule. J. Biol. Chem.238, 3188–3192 (1960).

    Google Scholar 

  14. Langstone, R., Distribution patterns of radioisotopes in plants. Proc. Am. Soc. Hort. Sci.68, 370–76 (1956).

    Google Scholar 

  15. Moeller, T., Inorganic Chemistry, an Advanced Textbook. J. Wiley, N. Y. 966 pp. (1952).

  16. Riceman, D. S. and Jones, G. B., Distribution of zinc in subterranean clover grown to maturity in culture solutions containing zinc labelled with the radioactive isotope Zn 65. Australian J. Agr. Research9, 730–744 (1958).

    Article  Google Scholar 

  17. Riceman, D. S. and Jones, G. B., Distribution of zinc and copper in subterranean clover (Trifolium subterraneum L.) in culture solutions supplied with dugraated amounts of zinc. Australian J. Agr. Research9, 73–122 (1958).

    Article  Google Scholar 

  18. Sayre, J. D., Accumulation of radioisotopes in corn leaves. Ohio Agr. Exp. Sta. Res. Bull. No 723. 30 pp. (1952).

  19. Sibly, P. M. and Wood, J. G., The nature of carbonic anhydrase from plant sources. Australian J. Sci. Research Ser. B-4, 500–510 (1951)

    Google Scholar 

  20. Skoog, F., Relationships between zinc and auxin in growth of higher plants. Am. J. Botany27, 939–951 (1940).

    Google Scholar 

  21. Stout, P. R., Micronutrients in crop vigor. J. Agr. Food Chem. 4, 100–106. (1956)

    Article  Google Scholar 

  22. Tsui, Ch., The role of zinc in auxin synthesis in the tomato plant. Am. J. Botany,35, 172–79 (1948).

    Google Scholar 

  23. Tupper, R., Watts, R. W. E., and Wormall, A., Some observations on the zinc in carbonic anhydrase. Biochem. J.50, 429–432 (1951).

    Google Scholar 

  24. Veldstra, H., The relation of chemical structure to biological activity in growth substance. Ann. Rev. Plant. Physiol.4, 151–198 (1953).

    Article  Google Scholar 

  25. Woltz, S., Toth, S. J., and Bear, F. E., Zinc status of New Jersey soils. Soil Sci.76, 115–122. (1953).

    Google Scholar 

  26. Wood, J. G. and Sibly, P. M., The distribution of zinc in oat plants. Australian J. Sci. Research Ser. B-3, 14–27 (1950).

    Google Scholar 

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Author notes

  1. Carmen Diez-Altares

    Present address: Universidad de Carabobo, Valencia, Venezuela

Authors and Affiliations

  1. Nuclear Energy Program Training and Research Center, Inter-American Institute of Agricultural Sciences of the OAS, Turrialba, Costa Rica

    Carmen Diez-Altares & Elemer Bornemisza

Authors
  1. Carmen Diez-Altares
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  2. Elemer Bornemisza
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Additional information

This work was supported by the U. S. Atomic Energy Commission, under Contract AT(30-1)-2043.

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Diez-Altares, C., Bornemisza, E. The localization of zinc-65 in germinating corn tissues. Plant Soil 26, 175–188 (1967). https://doi.org/10.1007/BF01978682

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  • DOI: https://doi.org/10.1007/BF01978682

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