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Identification of intracellular particles of pesticides in ciliate protozoa by Raman microprobe

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Summary

A Raman microprobe technique was used for the identification of intracellular particles of a pesticide added to a culture of the ciliate protozoanColpidium campylum. 2 methods, a wet and a dry, can be used for sample preparation. In both cases it was possible to find characteristic lines of 2 pollutants: 4′,4′-dichlorodiphenyl andβ-endosulfan. Some technical problems were encountered: the dry method causes drastic cellular alteration; the cell can move and go out of laser focus in the wet method.

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References

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Authors and Affiliations

  1. INSERM, U146, Domaine du CERTIA, 369, rue Jules Guesde, F-59650, Villeneuve d'Ascq, France

    D. Dive, J. M. Devynck, G. Leroy, M. N. Fourmaux & Y. Moschetto

  2. Centre de Technologie Biomédicale, INSERM, 13-17, rue Camille Guérin, F-59800, Lille, France

    D. Dive, J. M. Devynck, G. Leroy, M. N. Fourmaux & Y. Moschetto

Authors
  1. D. Dive
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  2. J. M. Devynck
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  3. G. Leroy
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  4. M. N. Fourmaux
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  5. Y. Moschetto
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Dive, D., Devynck, J.M., Leroy, G. et al. Identification of intracellular particles of pesticides in ciliate protozoa by Raman microprobe. Experientia 36, 832–834 (1980). https://doi.org/10.1007/BF01978597

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  • DOI: https://doi.org/10.1007/BF01978597

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