Netherlands Journal of Plant Pathology

, Volume 74, Supplement 1, pp 25–36 | Cite as

Protein synthesis by uredospores of the bean rust fungus

  • R. C. Staples
Article

Abstract

Ribosomes were isolated from dormant and germinated uredospores of the bean rust fungus and found to have sedimentation constants (S20, w) of 82.3. A supernatant solution of activating enzymes prepared from uredospores catalyzed amino acid incorporation by ribosomes. A small proportion of the ribosomes was associated in ribonuclease sensitive clusters. In the absence of added messenger RNA, a large proportion of the amino acid incorporation was carried out by these polysomes, which had sedimentation coefficients at least as high as 204 S. Presence of messenger RNA in spores is inferred from the fact that polysomes are present and that RNA preparations from spores at various stages of germination have template activity in an assay system prepared fromEscherichia coli.

Samenvatting

Uit rustende en gekiemde uredosporen van de boneroest,Uromyces appendiculatus (Pers.) Ung., (syn.U. phaseoli (Pers.) Wint.) werden ribosomen geïsoleerd met een sedimentatieconstante (S20,w) van 82.3. Incorporatie van aminozuren door de ribosomen werd gekatalyseerd door een oplossing van activerende enzymen, verkregen uit de uredosporen. Een klein deel van de ribosomen kwam voor in polysoomstructuren, die gevoelig waren voor ribonuclease. Wanneer geen boodschapper-RNA werd toegevoegd, was de aminozuur-incorporende activiteit grotendeels geassocieerd met deze polysomen, die sedimentatieconstanten hadden van tenminste 204 S. RNA, geïsoleerd uit sporen in verschillende stadia van de kieming, had boodschapperactiviteit, zoals bleek bij toetsing in een celvrij aminozuur-incorporerend systeem uitEscherichia coli. Hieruit en uit het feit, dat polysomen aanwezig waren, kan tot de aanwezigheid van boodschapper-RNA in de uredosporen geconcludeerd worden.

Keywords

Enzyme Sedimentation Germinate Protein Synthesis Assay System 

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1968

Authors and Affiliations

  • R. C. Staples
    • 1
  1. 1.Boyce Thompson Institute for Plant Research, Inc.YonkersU.S.A.

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