Abstract
TwoPenicillium species, vizP. brevicompactum andP. corymbiferum, were isolated from senescent petioles of cyclamen and from bulbs of lilies, respectively, both samples treated previously with benomyl. The isolates turned out to be very resistant to this fungicide when grown on malt agar, supplied with the fungicide; at a concentration of 2000 μg/ml they were less inhibited than randomly chosen isolates of the same species on agar with 1 μg/ml.
The strains retained their resistance at the same level for at least 3 months after repeated subculturing on fungicide-free agar.
Resistance to benomyl coincided with resistance to methyl-thiophanate and, to a lesser extent, also to thiabendazole and furidazol.
Samenvatting
Uit afstervende bladstelen van cyclamen en uit schubben van leliebollen, welke eerder met benomyl waren behandeld, konden respectievelijkPenicillium brevicompactum enPenicillium corymbiferum worden geïsoleerd. De isolaten blekenin vitro zeer resistent tegen het fungicide. De myceliumgroei van deze isolaten werd op moutagar met 2000 μg/ml benomyl minder geremd dan die van willekeurige isolaten van dezelfde soorten op agar met 1 μg/ml (Fig. 1).
De isolaten bleven gedurende tenminste 3 maanden resistent na regelmatig overenten op voedingsbodems zonder het fungicide.
De resistente stammen van de beidePenicillium-soorten bleken eveneens resistent tegen methyl-thiophanaat en in mindere mate ook tegen thiabendazol en furidazol (Tabel 1). De volgorde van de groeiremmende werking van deze fungiciden was voor de willekeurig gekozen (gevoelige) isolaten: benomyl>thiabendazol>methyl-thiophanaat >furidazol. Voor de resistente stammen was deze: thiabendazol en furidazol >benomyl>methyl-thiophanaat. In het feit dat een dergelijke verandering in volgorde van remmend effect ook voorBotrytis cinerea geldt, ligt een aanwijzing, dat de wijze waarop de resistentie werkt, voor deze schimmels gelijk is.
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Bollen, G.J. Resistance to benomyl and some chemically related compounds in strains of Penicillium species. Netherlands Journal of Plant Pathology 77, 187–193 (1971). https://doi.org/10.1007/BF01977277
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DOI: https://doi.org/10.1007/BF01977277