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Netherlands Journal of Plant Pathology

, Volume 81, Issue 4, pp 125–137 | Cite as

Comparative abilities of fungi pathogenic and nonpathogenic to bean (Phaseolus vulgaris) to metabolize phaseollin

  • J. Van den Heuvel
  • Judy A. Glazener
Article

Abstract

The abilities of fungi pathogenic and nonpathogenic to bean (Phaseolus vulgaris) to metabolize the phytoalexin phaseollin were compared when grown in shake cultures containing 12 to 15 μg phaseollin/ml. Under these conditions phaseollin was metabolized by five out of seven pathogens and by three out of five nonpathogens.

Disappearance of phaseollin was accompanied by the appearance of metabolic products in cultures ofFusarium solani f. sp.phaseoli, Colletotrichum lindemuthianum, Botrytis cinerea andCladosporium herbarum. The nonpathogenC. herbarum detoxified phaseollin to 1a-hydroxyphaseollone as rapidly as the pathogenF. solani f.sp.phaseoli. Phaseollin was converted to 6a-hydroxyphaseollin by the pathogensB. cinerea andC. lindemuthianum, and this product was further metabolized by the latter fungus. 6a-Hydroxyphaseollin was less fungitoxic toB. cinerea. C. lindemuthianum was equally sensitive to both compounds.

Phaseollin was not metabolized by the pathogensFusarium oxysporum f. sp.phaseoli andThielaviopsis basicola.

Samenvatting

Een vergelijkend onderzoek werd verricht naar het vermogen van schimmels, al dan niet pathogeen voor boon (Phaseolus vulgaris), om het fytoalexine phaseolline om te zetten.

In schudculturen waaraan 12 tot 15 μg phaseolline/ml was toegevoegd, kon phaseolline worden omgezet door vijf van de zeven onderzochte pathogenen en door drie van de vijf getoetste niet-pathogenen (Fig. 1, 2 en 3).

Het verdwijnen van phaseolline ging gepaard met het verschijnen van omzettings-produkten in culturen vanFusarium solani f. sp.phaseoli, Colletotrichum lindemuthianum, Botrytis cinerea enCladosporium herbarum. De niet-pathogene schimmelC. herbarum detoxificeerde phaseolline tot 1a-hydroxyphaseollon even snel als het pathogeenF. solani f. sp.phaseoli. Phaseolline werd omgezet tot 6a-hydroxyphaseolline door de pathogenenB. cinerea enC. lindemuthianum en dit produkt werd doorC. lindemuthianum weer verder omgezet tot een verwante verbinding. 6a-Hydroxyphaseolline was minder fungitoxisch dan phaseolline voorB. cinerea, maar even fungitoxisch voorC. lindemuthianum (Tabel 1).

Phaseolline werd niet omgezet door de bonepathogenenFusarium oxysporum f. sp.phaseoli enThielaviopsis basicola.

Geconcludeerd wordt dat er geen direkt verband bestaat tussen het vermogen van een schimmel tot omzetting of detoxificatie van phaseolline in vitro en zijn pathogeniteit voor boon.

Keywords

Plant Pathology Metabolic Product Botrytis Cinerea Shake Culture Colletotrichum Lindemuthianum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1975

Authors and Affiliations

  • J. Van den Heuvel
    • 1
  • Judy A. Glazener
    • 1
  1. 1.Phytopathological Laboratory ‘Willie Commelin Scholten’Baarnthe Netherlands

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