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Antagonism of Azotobacter chroococcum isolates to Rhizoctonia solani

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Abstract

Antagonism of isolates ofAzotobacter chroococcum toRhizoctonia solani on agar plates was studied, and isolates were tested for their ability to controlR. solani infection of potato sprouts in sterilized and unsterilized soil. The degree of antagonism exhibited varied strongly among the isolates and was also found to be temperature-dependent.

At 25, 20 and 15°C, all but one strongly antagonisticAzobacter isolate effectively prevented infection of sprouts of potatoes planted in a soil heavily infected with a pathogenic isolate ofR. solani. At 10°C none was effective.

Samenvatting

Op agarvoedingsbodems en in steriele en niet-steriele grond werd de antagonistische (antibiotische) activiteit van de bacterieAzotobacter chroococcum t.o.v. een pathogene stam vanRhizoctonia solani getoetst. In grond, een lichte zavel met pH 6,5 dienden spruiten van ‘Irene’ pootaardappelen als indicatoren voor de activiteit vanR. solani, waarmee de grond en de poters kunstmatig waren besmet.

De antagonistische activiteit van de isolaten vanA. chroococcum bleek op agar zeer sterk uiteen te lopen. Van de isolaten die op agar actief waren, bleek er één in grond bijna geen remmende activiteit te vertonen. In niet-steriele grond was het beeld gelijk aan dat van steriele grond: een snelle biologische inactivering van remstof(fen) trad dus niet op.

Er was een grote temperatuursinvloed. Bij 25, 20 en 15°C konden sterk antogonistische stammen vanA. chroococcum aantasting van aardappelspruiten doorR. solani afdoende voorkomen. Bij 10°C trad bijna geen bescherming op. Groei en stofwisseling vanA. chroococcum zijn dan sterk gereduceerd.

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Meshram, S.U., Jager, G. Antagonism of Azotobacter chroococcum isolates to Rhizoctonia solani. Netherlands Journal of Plant Pathology 89, 191–197 (1983). https://doi.org/10.1007/BF01976784

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