Netherlands Journal of Plant Pathology

, Volume 79, Issue 6, pp 249–256 | Cite as

Tomato spotted wilt virus in the anthers of Tropaeolum majus

  • T. S. Ie


Electron microscopy of ultrathin sections of anthers ofTropaeolum majus plants infected with tomato spotted wilt virus, revealed particles of the virus only in the cells of the endothecium tissue of the anthers. In these parenchyma cells the particles were localized, sometimes in high concentrations, only in the cytoplasm, in clusters in the cisternae of the endoplasmic reticulum. They were not present in the tapetal cells, nor in the pollen grains.


Bij elektronenmicroscopisch onderzoek van ultradunne coupes van helmknoppen van planten vanTropaeolum majus, geïnfecteerd met het tomatebronsvlekkenvirus (tomato spotted wilt virus TSWV), is waargenoment dat de virusdeeltjes uitsluitend voorkomen in de cellen van het endotheciumweefsel van de helmknop. In deze parenchymcellen zijn de virusdeeltjes beperkt tot het cytoplasma, en wel in groepjes in de cisternae van het endoplasmatisch reticulum (Fig. 2, 3 en 4). De virusdeeltjes komen echter niet voor in de cellen van de tapetumlaag, evenmin in de pollenmoedercellen of in de rijpe stuifmeelkorrels (Fig. 5, 6). Solitaire deeltjes (S-bodies) daarentegen, komen wel voor in alle cellen van de helmknop, evenals in de stuimeelkorrels van planten, die S-body-positief zijn (Fig. 6).


Microscopy Electron Microscopy Endoplasmic Reticulum Plant Pathology Parenchyma Cell 
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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1973

Authors and Affiliations

  • T. S. Ie
    • 1
  1. 1.Laboratory of VirologyState Agricultural UniversityWageningenthe Netherlands

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