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Purification of 20α-hydroxysteroid dehydrogenase from human erythrocytes

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Summary

20α-hydroxysteroid dehydrogenase was found in the supernatant after hemolysis of human erythrocytes, and the enzyme was isolated from the membrane-free hemolysate on a DEAE-cellulose column. The NADPH-generating system was essential for the reaction.

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Author notes

  1. M. Takeshita

    Present address: Department of Biochemistry, Medical College of Oita, Hazama-cho, 879-56, Oita, Japan

Authors and Affiliations

  1. Department of Biochemistry, Kanazawa University School of Medicine, 920, Kanazawa, Japan

    E. Shirasawa, M. Takeshita & Y. Yoneyama

Authors
  1. E. Shirasawa
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  2. M. Takeshita
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  3. Y. Yoneyama
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Additional information

Acknowledgments. We wish to thank Dr A. Tomoda, Department of Biochemistry, Kanazawa University School of Medicine, and Dr T. Yubisui, Department of Biochemistry, Medical College of Oita, for their advice and useful discussions.

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Shirasawa, E., Takeshita, M. & Yoneyama, Y. Purification of 20α-hydroxysteroid dehydrogenase from human erythrocytes. Experientia 36, 1159–1160 (1980). https://doi.org/10.1007/BF01976098

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