Inhibition of thiamine transport in baker's yeast by methylene blue
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Methylene blue was found to inhibit thiamine transport competitively (Ki=0.63 μM) in baker's yeast. The dye was also effective in abolishing the growth inhibition ofSaccharomyces cerevisiae by pyrithiamine which is known to be taken up by a common transport system for thiamine in yeast cells. A possible mechanism for the inhibition by methylene blue of the thiamine transport system in baker's yeast is discussed.
KeywordsMethylene Growth Inhibition Methylene Blue Yeast Cell Transport System
- 1.J. Suzuoki, J. Biochem.42, 27 (1955).Google Scholar