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Electrophoretic transport of Tl+ in mitochondria

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Summary

The distribution of Tl+ between rat liver mitochondria and the medium was studied; millimolar or smaller concentrations of Tl+ were labeled with204Tl. The Tl+ distribution responded to transient diffusion potentials in a way that indicated electrophoretic movements of Tl+. The diffusion potentials were induced by efflux of K+ in response to addition of valinomycin to nonrespiring mitochondria suspended in a medium with low concentrations of K+ or by efflux of H+ induced by making the medium more alkaline in the presence of a protonophorous (proton-conducting) uncoupling agent. Changes in membrane potential induced by valinomycin were followed with the aid of safranine. Tl+ brought about collapse of the diffusion potential. It is concluded that Tl+ is able to penetrate the mitochondrial membrane electrophoretically.

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Authors and Affiliations

  1. Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences, Leningrad, USSR

    Igor A. Skulskii, Margarita V. Savina, Vadim V. Glasunov & Nils-Erik L. Saris

  2. Department of Medical Chemistry, University of Helsinki, SF-00170, Helsinki, Finland

    Igor A. Skulskii, Margarita V. Savina, Vadim V. Glasunov & Nils-Erik L. Saris

Authors
  1. Igor A. Skulskii
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  2. Margarita V. Savina
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  3. Vadim V. Glasunov
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  4. Nils-Erik L. Saris
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Skulskii, I.A., Savina, M.V., Glasunov, V.V. et al. Electrophoretic transport of Tl+ in mitochondria. J. Membrain Biol. 44, 187–194 (1978). https://doi.org/10.1007/BF01976038

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  • DOI: https://doi.org/10.1007/BF01976038

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