Transgenic Research

, Volume 3, Issue 1, pp 43–49 | Cite as

Anthranilate synthase and chorismate mutase activities in transgenic tobacco plants overexpressing tryptophan decarboxylase fromCatharanthus roseus

  • Charlotte Poulsen
  • Oscar J. M. Goddijn
  • J. Harry C. Hoge
  • Robert Verpoorte


TransgenicNicotiana tabacum L. ‘Petit Havana’ SR1 F1-plants expressing tryptophan decarboxylase cDNA (tdc) fromCatharanthus roseus (L.) G. Don under the control of the CaMV 35S promoter and terminator exhibited tryptophan decarboxylase (TDC) enzyme activity and accumulated tryptamine. The plants with the highest TDC activity contained 19 pkat per mg of protein. The influence of transgenic expression oftdc on the activities of anthranilate synthase (AS) and chorismate mutase (CM) were examined in 10 transgenic tobacco plants. The specific activities of these two chorismate-utilizing enzymes were not significantly affected by expression oftdc, despite their important functions as branch point enzymes in the shikimate pathway. The results indicate that the normal route of tryptophan biosynthesis in plants is sufficient to supply a considerable amount of this essential amino acid for the biosynthesis of secondary metabolites. Despite their increased tryptamine content, the growth and development of the transgenic tobacco plants expressingtdc appeared normal.


tryptophan decarboxylase anthranilate synthase chorismate mutase transgenic tobacco 


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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Charlotte Poulsen
    • 1
    • 2
  • Oscar J. M. Goddijn
    • 3
  • J. Harry C. Hoge
    • 3
  • Robert Verpoorte
    • 2
  1. 1.Danisco A/SCopenhagen KDenmark
  2. 2.Project Group Plant Cell Biotechnology Biotechnology Delft Leiden, Center for Bio-Pharmaceutical Sciences Division of PharmacognosyUniversity of LeidenLeidenThe Netherlands
  3. 3.Institute of Molecular Plant Sciences, Clusius LaboratoryUniversity of LeidenLeidenThe Netherlands

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