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Synthetic activities of mass cultures and clones of human gingival fibroblasts

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Summary

The percentage of synthesis dedicated to collagen is elevated in low-density cultures of human gingival fibroblasts, as is per-cell total protein synthetic activity and glycosaminoglycan accumulation. These observations can be explained, in part, by a decrease in membrane transport of precursor substance in high-density cultures. Synthetic activity by human fibroblasts can be reliably assayed in vitro using as few as 500 cells sparsely seeded. Such low-cell number assay is essential for study of single-cell clones, where replicative life span is limited.

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Authors and Affiliations

  1. Department of Anatomy and Periodontics, University of Maryland, Dental School, 21201, Baltimore, Maryland, USA

    T. M. Hassell, D. V. Provenza & R. A. Foster

Authors
  1. T. M. Hassell
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  2. D. V. Provenza
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  3. R. A. Foster
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Additional information

Supported by National Institutes of Health grants DE-06671 and DE-05459. Dr Hassell is recipient of Research Career Development Award no. DE-00123 from the National Institute of Dental Research. We thank Ms N. C. Tappe for excellent technical assistance and Mrs Joy Cook for manuscript preparation.

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Hassell, T.M., Provenza, D.V. & Foster, R.A. Synthetic activities of mass cultures and clones of human gingival fibroblasts. Experientia 42, 66–69 (1986). https://doi.org/10.1007/BF01975899

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