Skip to main content
SpringerLink
Log in

Comparative cytochemical and pharmacological studies on the cholinergic innervation of the branchial heart of the cephalopodSepia officinalis (L.)

  • Full Papers
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The dense motor innervation of the obliquely striated muscle cells in the branchial heart of Coleoida is composed of activating and inhibiting parts. The inhibiting cholinergic system investigated in this study is characterized histo- and cytochemically by a high acetylcholinesterase activity (EC 3.1.1.7) in the glycocalices of the nerve fibers with transparent synaptic vesicles, the muscle cells and, to a lesser extent, the ovoid interstitial cells, the functions of which are endocytosis and storage of catabolic substances. The pharmacological results from the isolated organ indicate a more nicotinic type of Ach-receptor, which can be reversibly blocked by D-tubocurarine and α-bungarotoxin, but not with the same intensity by tetraethylammonium or atropine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Alexandrowicz, J.S., Innervation of the hearts ofSepia officinalis. Acta zool. Stockh.41 (1960) 65–100.

    Article  Google Scholar 

  2. Bacq, Z.M., Réactions du ventricule médian isolé deLoligo pealii à l'acétylcholin, à l'atropine et aux ions K, Ca et Mg. C. r. Soc. Biol.114 (1933) 1360–1361.

    CAS  Google Scholar 

  3. Bodian, D., The staining of paraffin sections of nervous tissues with activated protargol. Anat. Rec.69 (1937) 153–162.

    Article  CAS  Google Scholar 

  4. Chang, C.C., and Lee, C.J., Isolation of neurotoxins from the venom ofBungarus multicinctus and their modes of neuromuscular blocking action. Archs int. Pharmacodyn.144 (1963) 241–257.

    CAS  Google Scholar 

  5. Fumagalli, L., De-Renzis, G., and Miani, N., Acetylcholine receptors: number and distribution in intact and deafferented superior cervical ganglion of the rat. J. Neurochem.27 (1976) 47–52.

    Article  CAS  PubMed  Google Scholar 

  6. Ghiretti, F., Action of choline and acetylcholine on the isolated heart of gastropod and cephalopod molluscs. Archs Sci. biol.32 (1948) 239–251.

    CAS  Google Scholar 

  7. Johansen, K., and Huston, M.J., Effects of some drugs on the circulation system of the intact, non-anesthetized cephalopodOctopus dofleini. Comp. Biochem. Physiol.5 (1962) 177–184.

    Article  CAS  PubMed  Google Scholar 

  8. Karnovsky, M.J., and Roots, L., A ‘direct-coloring’ thiocholine method for choline-esterase. J. Histochem. Cytochem.12 (1964) 219–221.

    Article  CAS  PubMed  Google Scholar 

  9. Kasa, P., and Csillik, B., Electron microscopic localization of cholinesterase by a copper-lead-thiocholine technique. J. Neurochem.13 (1966) 1345–1349.

    Article  CAS  PubMed  Google Scholar 

  10. Katz, B., and Miledi, R., The effect of α-bungarotoxin on acetylcholine receptors. Br. J. Pharmac.49 (1973) 138–139.

    Article  CAS  Google Scholar 

  11. Kehoe, J.S., Sealock, R., and Bon, C., Effects of α-toxins fromBungarus multicinctus andB. caeruleus on cholinergic responses inAplysia neurones. Brain Res.107 (1976) 527–540.

    Article  CAS  PubMed  Google Scholar 

  12. Kling, G., Innervation und Pharmakologie des Zentralherzens vonSepia officinalis (L.). Verh. dt. zool. Ges.76 (1983) 298.

    Google Scholar 

  13. Kruta, V., Sur l'action de l'acetylcholine et de l'atropine sur la cœur deSepia officinalis. C. r. Soc. Biol.119 (1935) 608.

    CAS  Google Scholar 

  14. Leake, L.D., and Walker, R.J., Invertebrate neuropharmacology, p. 86. Blackie, Glasgow and London 1980.

    Google Scholar 

  15. Lee, C.Y., Tseng, L.F., and Chin, T.H., Influence of denervation in localization of neurotoxins from elapid venoms in rat diaphragm. Nature215 (1976) 1177–1178.

    Article  Google Scholar 

  16. Mislin, H., and Kauffmann, M., Der aktive Gefässpuls in der Arm-Schirmhaut der Cephalopoden. Revue suisse Zool.55 (1948) 267–271.

    Article  Google Scholar 

  17. Mislin, H., Über Beziehungen zwischen Atmung und Kreislauf bei Cephalopoden (Sepia officinalis L.). Synchronregistrierung von Elektrokardiogramm (EKG) und Atembewegung am schwimmenden Tier. Zool. Anz., Suppl.30 (1967) 175–181.

    Google Scholar 

  18. Schipp, R., and Schäfer, A., Vergleichende elektronenmikroskopische Untersuchungen an den zentralen Herzorganen von Cephalopoden. Feinstruktur des Herzens. Z. Zellforsch.98 (1969) 576–698.

    Article  CAS  PubMed  Google Scholar 

  19. Schipp, R., and Schäfer, A., Vergleichende elektronenmikroskopische Untersuchungen an den zentralen Herzorganen von Cephalopoden. Feinstruktur und Funktion der Kiemenherzen. Z. Zellforsch.101 (1969) 367–379.

    Article  CAS  PubMed  Google Scholar 

  20. Schipp, R., Schäfer, A., and Höhn, P., Elektronenmikroskopische und histochemische Untersuchungen zur Funktion des Kiemenherzanhanges (Pericardialdrüse) vonSepia officinalis. Z. Zellforsch.117 (1971) 252–274.

    Article  CAS  PubMed  Google Scholar 

  21. Schipp, R., and Hevert, F., Distribution of copper and iron in some central organs ofSepia officinalis (Cephalopoda). A comparative study by flameless atomic absorption and electron microscopy. Marine Biol.47 (1978) 391–399.

    Article  CAS  Google Scholar 

  22. Schipp, R., and Hevert, F., Ultrafiltration in the branchial heart appendage of dibranchiate cephalopods: a comparative ultrastructural and physiological study. J. exp. Biol.92 (1981) 23–35.

    Article  Google Scholar 

  23. Sundermann, G., Die Ultrastruktur der vakuolisierten Rundzellen vonLoligo vulgaris Lam. (Mollusca, Cephalopoda). Zool. Jb. Anat.103 (1980) 93–104.

    Google Scholar 

  24. Wells, M.J., and Mangold, K., The effects of extracts from neurosecretory cells in the anterior vena cava and pharyngoophthalmic vein upon the hearts of intact free-moving octopuses. J. exp. Biol.84 (1980) 319–334.

    Article  CAS  PubMed  Google Scholar 

  25. Wells, M.J., Nervous control of the heartbeat inOctopus. J. exp. Biol.85 (1980) 111–128.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Allgemeine und Spezielle Zoologie, Justus Liebig University, D-6300, Giessen, Federal Republic of Germany

    R. Schipp, H. R. Schmidt & A. Fiedler

  2. Station Biologique d'Arcachon, F-33120, Arcachon, France

    R. Schipp, H. R. Schmidt & A. Fiedler

Authors
  1. R. Schipp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. R. Schmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Fiedler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schipp, R., Schmidt, H.R. & Fiedler, A. Comparative cytochemical and pharmacological studies on the cholinergic innervation of the branchial heart of the cephalopodSepia officinalis (L.). Experientia 42, 23–30 (1986). https://doi.org/10.1007/BF01975878

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01975878

Key words

Search

Navigation