Comparison of different culture media for isolation ofChlamydia trachomatis by cell culture on HeLa cells

  • P. Herbrink
  • M. Zuyderwijk-Zwinkels
  • B. Niesters
  • J. Wagenvoort
  • W. Van Dijk


The isolation yield and number ofChlamydia trachomatis inclusions was compared using DEAE-dextran pretreated HeLa cells cultured in four different media: Eagle's minimal essential medium (EMEM) with and without cycloheximide and Dulbecco's modification of EMEM (DMEM) with and without cycloheximide. Using DMEM without cycloheximide the number of inclusions was significantly higher than using EMEM without cycloheximide. In addition, the size of the inclusions was greatly enhanced. Use of DMEM or EMEM with cycloheximide yielded results comparable to those obtained with DMEM without cycloheximide.


Cell Culture Internal Medicine HeLa Cell Minimal Essential Medium Cycloheximide 
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  1. 1.
    Ridgway GL The laboratory diagnosis of chlamydial infections. In: Oriel D, Ridgeway G, Schachter J, Taylor-Robinson D, Ward M (ed): Chlamydial infections. Cambridge University Press, Cambridge, 1986, p. 539–549.Google Scholar
  2. 2.
    Gordon FF, Quan AL Isolation of the trachoma agent in cell culture. Proceedings of the Society for Experimental Biology and Medicine 1965, 118: 354–359.PubMedGoogle Scholar
  3. 3.
    Kuo CC, Grayston JT Interaction ofChlamydia trachomatis organisms and HeLa 229 cells. Infection and Immunity 1976, 13: 1103–1109.PubMedGoogle Scholar
  4. 4.
    Kuo CC, Wang SP, Wentworth BB, Grayston JT Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran. Journal of Infectious Diseases 1972, 125: 665–668.PubMedGoogle Scholar
  5. 5.
    Benes S, McCormack WM Comparison of methods for cultivation and isolation ofChlamydia trachomatis. Journal of Clinical Microbiology 1982, 16: 847–850.PubMedGoogle Scholar
  6. 6.
    Ripa KT, Mardh PA Cultivation ofChlamydia trachomatis in cycloheximide treated McCoy cells. Journal of Clinical Microbiology 1977, 6: 328–331.PubMedGoogle Scholar
  7. 7.
    Wagenvoort JTH, van Rijsoort-Vos T, Overkleeft-van de Ree A, Stolz E Enhancement of yield ofChlamydia trachomatis HeLa 229 cell culture. European Journal of Clinical Microbiology and Infectious Diseases 1988, 7: 822.Google Scholar
  8. 8.
    Sabet SF, Simmons J, Caldwell HC Enhancement ofChlamydia trachomatis infectious progeny by cultivation in HeLa 229 cells treated with DEAE-dextran and cycloheximide. Journal of Clinical Microbiology 1984, 20: 217–222.PubMedGoogle Scholar
  9. 9.
    Ennis HL, Lubin M Cycloheximide: aspects of inhibition of protein synthesis in mammalian cells. Science 1964, 146: 1474–1476.PubMedGoogle Scholar
  10. 10.
    Alexander JJ Separation of protein synthesis in mening-opneumonitis agent from that in L cells by differential susceptibility to cycloheximide. Journal of Bacteriology 1968, 95: 327–332.PubMedGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1991

Authors and Affiliations

  • P. Herbrink
    • 1
    • 3
  • M. Zuyderwijk-Zwinkels
    • 2
  • B. Niesters
    • 2
  • J. Wagenvoort
    • 4
  • W. Van Dijk
    • 2
  1. 1.Department of Research and DevelopmentDiagnostic Centre SSDZDelftThe Netherlands
  2. 2.Department of Clinical MicrobiologyDiagnostic Centre SSDZDelftThe Netherlands
  3. 3.Department of DermatovenerologyUniversity HospitalRotterdamThe Netherlands
  4. 4.Department of Clinical MicrobiologyUniversity HospitalRotterdamThe Netherlands

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