Comparison of different culture media for isolation ofChlamydia trachomatis by cell culture on HeLa cells

  • P. Herbrink
  • M. Zuyderwijk-Zwinkels
  • B. Niesters
  • J. Wagenvoort
  • W. Van Dijk
Notes

Abstract

The isolation yield and number ofChlamydia trachomatis inclusions was compared using DEAE-dextran pretreated HeLa cells cultured in four different media: Eagle's minimal essential medium (EMEM) with and without cycloheximide and Dulbecco's modification of EMEM (DMEM) with and without cycloheximide. Using DMEM without cycloheximide the number of inclusions was significantly higher than using EMEM without cycloheximide. In addition, the size of the inclusions was greatly enhanced. Use of DMEM or EMEM with cycloheximide yielded results comparable to those obtained with DMEM without cycloheximide.

Keywords

Cell Culture Internal Medicine HeLa Cell Minimal Essential Medium Cycloheximide 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1991

Authors and Affiliations

  • P. Herbrink
    • 1
    • 3
  • M. Zuyderwijk-Zwinkels
    • 2
  • B. Niesters
    • 2
  • J. Wagenvoort
    • 4
  • W. Van Dijk
    • 2
  1. 1.Department of Research and DevelopmentDiagnostic Centre SSDZDelftThe Netherlands
  2. 2.Department of Clinical MicrobiologyDiagnostic Centre SSDZDelftThe Netherlands
  3. 3.Department of DermatovenerologyUniversity HospitalRotterdamThe Netherlands
  4. 4.Department of Clinical MicrobiologyUniversity HospitalRotterdamThe Netherlands

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