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Presumptive identification ofSalmonella enterica using two rapid tests

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Abstract

Two rapid test for early detection ofSalmonella enterica, the cytochrome oxidase test and a fluorescence test, were evaluated in 1,200 colonies which had been isolated from human feces and cultured on MacConkey and Salmonella-Shigella media. Using the fluorescence test there were no false negative results (sensitivity 100 %) and of 205 positive cases 62 did not correspond biochemically to salmonella (specificity 94.1 %); 44 of these 62 were positive in the cytochrome oxidase test, raising the specificity to 98.2 %. In conclusion, the combination of the two tests could be very useful due to the ease of performance, low cost and excellent results obtained.

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Authors and Affiliations

  1. Seccion de Microbiología, Hospital “Virgen de la Arrixaca”, El Palmar, 30120, Murica, Spain

    J. Ruiz, M. C. Varela, M. A. Sempere & M. L. Lopez

  2. Servicio de Medicina Interna, Hospital “Virgen de la Arrixaca”, El Palmar, 30120, Murcia, Spain

    J. Gomez

  3. Laboratorio del Centro de Nuevas Tecnologías, Murcia, Spain

    J. Oliva

Authors
  1. J. Ruiz
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  2. M. C. Varela
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  3. M. A. Sempere
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  4. M. L. Lopez
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  5. J. Gomez
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  6. J. Oliva
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Ruiz, J., Varela, M.C., Sempere, M.A. et al. Presumptive identification ofSalmonella enterica using two rapid tests. Eur. J. Clin. Microbiol. Infect. Dis. 10, 649–651 (1991). https://doi.org/10.1007/BF01975818

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  • DOI: https://doi.org/10.1007/BF01975818

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