Specificity of new peptide immunoassays versus other immunoassays and agglutination tests for detection of HIV antibody in African Sera

  • G. Léonard
  • M. Verdier
  • A. Sangaré
  • J. L. Rey
  • F. Denis


The specificity of three HIV EIAs using synthetic peptides was evaluated and compared with that of four commercial EIAs using recombinant proteins or whole virus lysates of HIV-1 or of HIV-1 and HIV-2, and of two tests based on agglutination of sensitized particles. A total of 734 West African sera were screened. The best results were obtained with tests using recombinant proteins. HIV tests using synthetic peptides able to distinguish between HIV-1 and HIV-2 antibodies showed comparable specificity, but some false negative results represent a major handicap of these peptide EIAs in screening for HIV antibodies.


Peptide Internal Medicine Recombinant Protein Synthetic Peptide False Negative Result 
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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1991

Authors and Affiliations

  • G. Léonard
    • 1
  • M. Verdier
    • 1
  • A. Sangaré
    • 2
  • J. L. Rey
    • 3
  • F. Denis
    • 1
  1. 1.Département de VirologieCentre Hospitalier Universitaire DupuytrenLimoges CedexFrance
  2. 2.Institut PasteurAbidjanIvory Coast
  3. 3.Organisme de Recherche Scientifique et Technique d'Outre MerMontpellierFrance

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