Advertisement

Specificity of new peptide immunoassays versus other immunoassays and agglutination tests for detection of HIV antibody in African Sera

  • G. Léonard
  • M. Verdier
  • A. Sangaré
  • J. L. Rey
  • F. Denis
Article

Abstract

The specificity of three HIV EIAs using synthetic peptides was evaluated and compared with that of four commercial EIAs using recombinant proteins or whole virus lysates of HIV-1 or of HIV-1 and HIV-2, and of two tests based on agglutination of sensitized particles. A total of 734 West African sera were screened. The best results were obtained with tests using recombinant proteins. HIV tests using synthetic peptides able to distinguish between HIV-1 and HIV-2 antibodies showed comparable specificity, but some false negative results represent a major handicap of these peptide EIAs in screening for HIV antibodies.

Keywords

Peptide Internal Medicine Recombinant Protein Synthetic Peptide False Negative Result 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Barré-Sinoussi F, Chermann JC, Rey F, Nugeyre MT, Chamaret S, Gruest J, Daugut C, Axler-Blin C, Brun-Vezinet F, Rouzioux C, Rozenbaum W, Montagnier L Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome. Science 1983, 220: 868–871.PubMedGoogle Scholar
  2. 2.
    Clavel F, Guétard D, Brun-Vezinet F, Chamaret S, Rey MA, Santos-Ferreira MO, Laurent AG, Daugut C, Katlama C, Rouzioux C, Klatzman D, Champalimaud JL, Montagnier L Isolation of a new retrovirus from West African patients with AIDS. Science 1986, 233: 343–346.PubMedGoogle Scholar
  3. 3.
    Gnann JW, McCormick JB, Mitchell S, Nelson JA, Oldstone MBA Synthetic peptide immunoassay distinguishes HIV type 1 and HIV type 2 infections. Science 1987, 237: 1346–1349.PubMedGoogle Scholar
  4. 4.
    Smith RS, Naso RB, Rosen J, Whalley A, Hom YL, Hoey K, Kennedy CJ, McCutchan JA, Spector SA, Richman DD Antibody to a synthetic oligopeptide in subjects at risk for human immunodeficiency virus infection. Journal of Clinical Microbiology 1987, 25: 1498–1504.PubMedGoogle Scholar
  5. 5.
    Norrby E, Biberfeld G, Chiodi F, Von Gegerfeldt A, Nauclear A, Parks E, Lerner R Discrimination between antibodies to HIV and to related retroviruses using site-directed serology. Nature 1987, 329: 248–250.CrossRefPubMedGoogle Scholar
  6. 6.
    Gnann JW, Schwimmbeck PL, Nelson JA, Truax AB, Oldstone MBA Diagnosis of AIDS by using a 12-amino acid peptide representing an immunodominant epitope of the human immunodeficiency virus. Journal of Infectious Diseases 1987, 156: 261–267.PubMedGoogle Scholar
  7. 7.
    Ayres L, Avillez F, Garcia-Benito A, Deinhardt F, Gürtler L, Denis F, Léonard G, Ranger S, Grob P, Joller-Jemelka H, Hess G, Seidl S, Flacke H, Simon F, Brun-Vezinet F, Sondag D, André A, Hampl H, Schoen R, Stramer S, Troonen H Multicenter evaluation of a new recombinant enzyme immunoassay for the combined detection of antibody to HIV-1 and HIV-2. AIDS 1990, 4: 131–138.PubMedGoogle Scholar
  8. 8.
    Biggar RJ, Gigase PL, Melbeye M, Elbesen P, Mann DL, Goedert JJ, Weinstock R, Strong D, Blattner WA ELISA HTLV retrovirus antibody reactivity associated with malaria and immunocomplexes in healthy Africans. Lancet 1985, ii: 520–523.CrossRefGoogle Scholar
  9. 9.
    Thorn RM, Beltz GA, Hung CH, Fallis BA, Winckle S, Cheng KL, Marciani DJ Enzyme immunoassay using a novel recombinant polypeptide to detect human immunodeficiency virusenv antibody. Journal of Clinical Microbiology 1987, 25: 1207–1212.PubMedGoogle Scholar
  10. 10.
    Denis F, Léonard G, Sangaré A, Gershy-Damet GM, Rey JL, Soro B, Schmidt D, Mounier M, Verdier M, Baillou A, Barin F Comparison of ten enzyme immunoassays for detection of antibody to human immunodeficiency virus type 2 in West African sera. Journal of Clinical Microbiology 1988, 26: 1000–1004.PubMedGoogle Scholar
  11. 11.
    Barin F, Denis F, Baillou A, Léonard G, Mounier M, M'Boup S, Gershy-Damet GM, Sangaré A, Kanki PJ, Essex M A STLV-III related human retrovirus HTLV-IV: analysis of cross-reactivity with the human immunodeficiency virus. Journal of Virological Methods 1987, 17: 55–61.CrossRefPubMedGoogle Scholar
  12. 12.
    Rayfield M, De Cock K, Heyward W, Goldstein L, Krebs J, Kwok S, Lee S, McCormick J, Moreau JM, Odehouri K, Schochetman G, Sninky J Mixed human immunodeficiency virus infection in an individual: demonstration of both HIV type 1 and type 2 proviral sequences by using polymerase chain reaction. Journal of Infectious Diseases 1988, 158: 1170–1176.PubMedGoogle Scholar
  13. 13.
    Evans LA, Moreau J, Odehouri K, Seto D, Thomson-Honnebier G, Legg H, Barboza A, Cheng-Mayer C, Levy JA Simultaneous isolation of HIV-1 and HIV-2 from an AIDS patient. Lancet 1988, ii: 1389–1392.CrossRefGoogle Scholar
  14. 14.
    Quinn TC, Riggin CH, Kline RL, Francis H, Mulanga K, Sension MG, Fauci AS Rapid latex agglutination assay using recombinant envelope polypeptide for the detection of antibody to the HIV. Journal of the American Medical Association 1988, 260: 510–513.CrossRefPubMedGoogle Scholar
  15. 15.
    Van de Perre P, Nzaramba D, Allen S, Riggin CH, Sprecher-Goldberger S, Butzler JP Comparison of six serological assays for human immunodeficiency virus antibody detection in developing countries. Journal of Clinical Microbiology 1988, 26: 552–556.PubMedGoogle Scholar
  16. 16.
    Alizon M, Wain-Hobson S, Montagnier L, Sonigo P Genetic variability of the AIDS virus: nucleotide sequence analysis of two isolates from African patients. Cell 1986, 46: 63–74.CrossRefPubMedGoogle Scholar
  17. 17.
    Hahn BH, Gonda MA, Shaw GM, Popovic M, Hoxie JA, Gallo RC, Wong-Staal F Genomic diversity of the acquired immune deficiency syndrome virus HTLV-III: different viruses exhibit greatest divergence in their envelope genes. Proceedings of the National Academy of Sciences of the USA 1985, 82: 4813–4817.PubMedGoogle Scholar
  18. 18.
    Franchini G, Fargnoli KA, Giombini F, Jagodzinski L, De Rossi A, Bosch M, Biberfeld G, Fenyo M, Albert J, Gallo RC, Wong-Staal F Molecular and biological characterization of a replication competent human immunodeficiency type 2 (HIV-2) proviral clone. Proceedings of the National Academy of Sciences of the USA 1989, 86: 2433–2437.PubMedGoogle Scholar
  19. 19.
    Kumar P, Hui H, Kappes JC, Haggarty BS, Hoxie JA, Arya SK, Shaw GM, Hahn BH Molecular characterization of an attenuated human immunodeficiency virus type 2 isolate. Journal of Virology 1990, 64: 890–901.PubMedGoogle Scholar
  20. 20.
    De Leys R, Vanderborght B, Vanden Haesevelde M, Heyndrickx L, Van Geel A, Wauters C, Bernaerts R, Saman E, Nijs P, Willems B, Taelman H, Van Der Groen G, Piot P, Tersmette T, Huisman JG, Van Heuverswyn H Isolation and partial characterization of an unusual human immunodeficiency retrovirus from two persons of West-Central African origin. Journal of Virology 1990, 64: 1207–1216.PubMedGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1991

Authors and Affiliations

  • G. Léonard
    • 1
  • M. Verdier
    • 1
  • A. Sangaré
    • 2
  • J. L. Rey
    • 3
  • F. Denis
    • 1
  1. 1.Département de VirologieCentre Hospitalier Universitaire DupuytrenLimoges CedexFrance
  2. 2.Institut PasteurAbidjanIvory Coast
  3. 3.Organisme de Recherche Scientifique et Technique d'Outre MerMontpellierFrance

Personalised recommendations