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Independent effects of interleukin 1 on proteoglycan synthesis and proteoglycan breakdown of bovine articular cartilagein vitro

  • Bone and Cartilage
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Abstract

We studied the effects of human recombinant interleukin-1β on proteoglycan metabolism of bovine articular cartilage in organ culture. IL-1 was more potent in inhibiting synthesis (IC50 4 ng/mL) than in stimulating breakdown of proteoglycans (EC50 200 ng/mL). Inhibition of proteoglycan synthesis began to plateau earlier (2 days) than stimulation of proteoglycan release (4 days). Both effects could be neutralized with a polyclonal anti-IL-1β antibody; however, higher antibody titers were required to block IL-1 effects on proteoglycan synthesis than to neutralize those on proteoglycan release. Chloroquine, but not hydrocortisone, blocked IL-1-mediated proteoglycan breakdown. Both drugs, however, augmented IL-1-induced inhibition of proteoglycan synthesis. Our data suggest that the effects of IL-1 on articular cartilage proteoglycan synthesis and proteoglycan breakdown can be regulated independently.

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Abbreviations

CS:

chondroitin sulfate

CPC:

cetylpyridinium chloride

DMEM:

Dulbecco's modified Eagle's medium

DMSO:

dimethyl sulfoxide

GAG:

glucosaminoglycan

PBS:

phosphate-buffered saline

RA:

rheumatoid arthritis

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  1. Articular Cartilage Research Laboratory, University Department of Orthopaedics, J. Stelzmann-Str. 9, D-50937, Köln, Germany

    J. Neidel & U. Zeidler

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Neidel, J., Zeidler, U. Independent effects of interleukin 1 on proteoglycan synthesis and proteoglycan breakdown of bovine articular cartilagein vitro . Agents and Actions 39, 82–90 (1993). https://doi.org/10.1007/BF01975718

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