Netherlands Journal of Plant Pathology

, Volume 75, Issue 6, pp 321–328 | Cite as

Production of tobacco mosaic virus in and its infectivity from leaves of two Nicotiana species treated with 6-azauracil

  • Jeanne Dijkstra


A correlation between the amount of virus as determined by infectivity test and that determined by a serological-spectrophotometric method was established in leaf discs ofNicotiana tabacum ‘White Burley’ which had floated on a solution of 6-azauracil (AzU) before and after inoculation with tobacco mosaic virus. More virus, determined in the two different ways, was present in the AzU-than in the water-treated leaf discs at 24 and 48 h after inoculation. Thereafter, the amount of virus was less than that in the control. The inhibitory effect of the pyrimidine analogue on the amount of infectivity in sap from AzU-treated discs proved to be dependent on the dilution of sap used. The higher the dilution the more inhibition occurred.

In inoculated leaf discs ofNicotiana glutinosa the formation of local lesions in the AzU-treated series was strongly inhibited at 48 h after inoculation whereas at that time the amount of virus determined by the serological-spectrophotometric method was higher than that in the water-treated control ones. The latter mentioned stimulating effect disappeared later than 48 h after inoculation and gave way to inhibition.

The infectivity of tobacco mosaic virus from AzU-treated leaf discs of ‘White Burley’ tobacco was 23% of that from water-treated controls when undiluted clarified sap was used.


Plant Pathology Pyrimidine Mosaic Virus Leaf Disc Tobacco Mosaic Virus 


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1969

Authors and Affiliations

  • Jeanne Dijkstra
    • 1
  1. 1.Laboratorium voor Virologie der LandbouwhogeschoolWageningen

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