Effects of cilofungin (LY121019) on carbohydrate and sterol composition ofCandida albicans
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Cilofungin (LY121019) is a novel analogue of echinocandin B with potent activity againstCandida albicans. The effects of cilofungin on the sterol and cell wall carbohydrate composition ofCandida albicans were investigated. Exposure ofCandida albicans to cilofungin resulted in a 55–60% decrease in ergosterol and a 4–13% decrease in lanosterol content relative to controls. Carbohydrate analysis revealed a 72–79% decrease in glucan content and no significant decrease in mannan or chitin content relative to controls. These studies suggest that cilofungin specifically inhibits glucan synthesis inCandida albicans and thus may be less toxic to mammalian cells. The effects of cilofungin on sterol composition may be non-specific and will require additional investigation.
KeywordsCarbohydrate Chitin Glucan Ergosterol Additional Investigation
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- 6.Dische, Z. Color reactions of hexosamines. In: Whistler, R. L., Wolfrom, M. Z. (ed.): Methods in carbohydrate chemistry. Academic Press, New York, 1962, p. 507–512.Google Scholar
- 7.Roberts, R. L., Blowers, B., Slater, M. L., Cabib, E. Chitin synthesis and localization in cell division cycle mutants ofSaccharomyces cerevisiae. Molecular and Cell Biology 1983, 3: 922–930.Google Scholar
- 8.Vanden Bossche, H. Biochemical targets for antifungal azole derivatives: hypothesis on the mode of action. In: McGinnis, M. R. (ed.): Current topics in medical mycology, Volume 1. Springer Verlag, New York, 1985, p. 313–351.Google Scholar
- 9.Rogers, H. J., Perkins, H. R., Ward, J. B. Biosynthesis of cell walls and membranes. Chapman and Hall, London, 1980, p. 478–507.Google Scholar
- 10.Gooday, G. W. The enzymology of hyphal growth. In: Smith, J. E., Berry, D. R. (ed.): The filamentous fungi: developmental biology, Volume 3. Edward Arnold, London, 1978, p. 51–77.Google Scholar