Advertisement

Experientia

, Volume 36, Issue 4, pp 395–396 | Cite as

Collagen breakdown by gingival collagenase and elastase

  • Y. Kowashi
  • G. Cimasoni
  • J. Matter
Article

Summary

The granule fraction of human polymorphonuclear leucocytes (PMNs), the concentrated product of gingival washing from 2 human volunteers and the culture fluid of samples of human gingiva were incubated with neutral salt soluble collagen from rat skin and the patterns of collagen degradation were studied by SDS polyacrylamide gel electrophoresis. Collagenase from human gingiva cleaved the collagen molecules in a fashion similar to that of the PMN granule fraction. Collagen was also attacked by elastase from human PMNs and, to a lesser extent, by elastase from the gingival washings.

Keywords

Malachite Green Culture Fluid Collagen Molecule Archs Oral Biol Disodium Ethylenediaminetetraacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Literatur

  1. 3.
    R. Attström and J. Egelberg, J. periodont. Res.5, 48 (1970).CrossRefPubMedGoogle Scholar
  2. 4.
    C. R. Schiøtt and H. Löe, J. periodont. Res.5, 36 (1970).CrossRefPubMedGoogle Scholar
  3. 5.
    A. Tzamouranis, J. Matthys, I. Ishikawa and G. Cimasoni, Archs oral Biol.22, 375 (1977).CrossRefGoogle Scholar
  4. 6.
    D.C. Wright and S.E. Malawista, J. Cell Biol.58, 788 (1972).CrossRefGoogle Scholar
  5. 7.
    K. Hasegawa, G. Cimasoni and P. Vuagnat, Experientia31, 765 (1975).CrossRefPubMedGoogle Scholar
  6. 8.
    Y. Kowashi, F. Jaccard and G. Cimasoni, Archs oral Biol.24, 645 (1980).CrossRefGoogle Scholar
  7. 9.
    G. Murphy, J.J. Reynolds, U. Bretz and M. Baggiolini, Biochem. J.162, 195 (1977).CrossRefPubMedPubMedCentralGoogle Scholar
  8. 10.
    I. Ishikawa and G. Cimasoni, Biochim. biophys. Acta480, 228 (1977).CrossRefPubMedGoogle Scholar
  9. 11.
    I. Ishikawa and G. Cimasoni, Archs oral Biol.23, 933 (1978).CrossRefGoogle Scholar
  10. 12.
    G. Cimasoni, in: Monographs in Oral Science, p. 43. Ed. H. Myers. Karger, Basel 1974.Google Scholar
  11. 13.
    H. Eagle, Science130, 432 (1959).CrossRefPubMedGoogle Scholar
  12. 14.
    R.B. Donoff, J.E. McLennan and H.C. Grillo, Biochim. biophys. Acta227, 639 (1971).CrossRefPubMedGoogle Scholar
  13. 15.
    A.H. Kang, Y. Nagai, K.A. Piez and J. Gross, Biochemistry5, 509 (1966).CrossRefPubMedGoogle Scholar
  14. 16.
    P.M. Starkey and A.J. Barrett, Biochem. J.155, 265 (1976).CrossRefPubMedPubMedCentralGoogle Scholar
  15. 17.
    V.J. Uitto, H. Turto and L. Saxen, J. periodont. Res.13, 207 (1978).CrossRefPubMedGoogle Scholar
  16. 18.
    G.S. Lazarus, J.R. Daniels, J. Lian and M.C. Burleigh, Am. J. Path.69, 565 (1972).Google Scholar
  17. 19.
    J. Gross and Y. Nagai, Proc. natn. Acad. Sci. USA54, 1197 (1965).CrossRefGoogle Scholar
  18. 20.
    A.Z. Eisen, J.J. Jeffrey and J. Gross, Biochim. biophys. Acta151, 637 (1968).CrossRefPubMedGoogle Scholar
  19. 21.
    P.M. Starkey, A.J. Barrett and M.C. Burleigh, Biochim. biophys. Acta483, 386 (1977).CrossRefPubMedGoogle Scholar
  20. 22.
    E.D. Harris and E.C. Cartwright, in: Proteinases in Mammalian Cells and Tissues, p. 249. Ed. A.J. Barrett, North Holland, Amsterdam 1977.Google Scholar
  21. 23.
    Z. Werb, in: Dynamics of Connective Tissue Macromolecules, p. 159. Ed. P.M.C. Burleigh and A.R. Poole. North Holland, Amsterdam 1975.Google Scholar

Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • Y. Kowashi
    • 1
  • G. Cimasoni
    • 1
  • J. Matter
    • 1
  1. 1.Dental SchoolGenève 4Switzerland

Personalised recommendations