Archives of Toxicology

, Volume 64, Issue 8, pp 616–622 | Cite as

Teratogenicity of arotinoids (retinoids) in vivo and in vitro

  • Andreas Kistler
  • Toshie Tsuchiya
  • Masahiko Tsuchiya
  • Michael Klaus
Original Investigations


The effect of structural modifications on the arotinoid molecule, a new class of retinoids, on their teratogenicity in mice was studied. Animals were treated on days 8 and 9 of gestation, the most susceptible stages to retinoid-induced malformations in rodents. The teratogenic potency of the 13 arotinoids tested varied over a dose range of more than five orders of magnitude. Next, we tested whether the quantitative differences in the teratogenicity of these arotinoids correlates with their activity in high density (micromass) cultures of rat embryonic limb bud and midbrain cells. There was a good quantitative correlation between the in vivo teratogenicity and the in vitro activity in limb bud cells but no correlation was found in midbrain cells. Thus, the limb bud cell culture system may be useful for a preliminary testing to select non-teratogenic retinoids. For the risk assessment in humans, however, the in vitro results should be verified in animals studies.

Key words

Teratogenicity in vivo Teratogenicity in vitro Retinoids Arotinoids Micromass culture 


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Andreas Kistler
    • 1
  • Toshie Tsuchiya
    • 2
  • Masahiko Tsuchiya
    • 1
  • Michael Klaus
    • 1
  1. 1.Phamaceutical ResearchF. Hoffmann-La Roche LtdBasel
  2. 2.Faculty of Veterinary MedicineUniversity of BerneBernSwitzerland

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