Beta-lactam resistance amongEscherichia coli andKlebsiella species blood culture isolates in Finnish hospitals

  • T. Arstila
  • H. Auvinen
  • P. Huovinen
  • the Finnish Study Group for Antimicrobial Resistance
Article

Abstract

The aim of this study was to investigate beta-lactam resistance inEscherichia coli andKlebsiella spp. blood culture isolates in Finland. Special attention was given to extended-spectrum beta-lactamases. A total of 566Escherichia coli and 108Klebsiella spp. blood culture isolates were collected from hospitals throughout Finland and their susceptibility to beta-lactam antibiotics studied. Twenty percent ofEscherichia coli and 69 % ofKlebsiella spp. strains were resistant to ampicillin. The mechanisms of resistance were studied by hybridization, isoelectric focusing and the clavulanate double-disk potentiation test. Of the ampicillin-resistantEscherichia coli strains, 83 % produced TEM-1. Of the ampicillin-resistantKlebsiella spp. strains, 43 % produced SHV-1. Only nineEscherichia coli and threeKlebsiella spp. isolates were resistant to cefuroxime (MIC ≥32 µg/ml), and none were resistant to third-generation cephalosporins. These data were compared with cefuroxime and third-generation cephalosporin consumption levels in Finnish hospitals. Although the use of cephalosporins is far more extensive in Finland than in other Scandinavian countries, none of the isolates produced extended-spectrum beta-lactamases. In conclusion, resistance to cefuroxime has remained rare in Finland, and cefuroxime is still an alternative to third-generation cephalosporins in the treatment of septicemia.

Keywords

Internal Medicine Ampicillin Blood Culture Cephalosporin Cefuroxime 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • T. Arstila
    • 1
  • H. Auvinen
    • 2
  • P. Huovinen
    • 1
  • the Finnish Study Group for Antimicrobial Resistance
  1. 1.Antimicrobial Research LaboratoryNational Public Health InstituteTurkuFinland
  2. 2.Kanta-Häme Central HospitalHämeenlinnaFinland

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