, Volume 45, Issue 8, pp 759–762 | Cite as

Necessity of adjuvants for inducing effective antibody response to zona pellucida antigens

  • P. Bhatnagar
  • S. Sehgal
  • S. K. Gupta
  • B. S. Dunbar
Research Articles


Rhesus monkeys evoked a vigorous antibody response to a single heteroimmunization dose of zona pellucida antigens, when these were administered along with complete Freud's adjuvant. The antisera recognized all the three major porcine zona glycoprotein families and the animals were rendered amenorrheic after such immunization. Monkeys immunized with zona without adjuvant, however, failed to show any anti-zona antibody response and had normal menstrual cycles. Zona pellucida glycoproteins are thus not effective immunogens unless administered along with a powerful adjuvant.

Key words

Zona pellucida glycoproteins adjuvant immunoblotting enzyme-linked immunosorbent assay autoantibodies infertility 


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Copyright information

© Birkhäuser Verlag 1989

Authors and Affiliations

  • P. Bhatnagar
    • 1
  • S. Sehgal
    • 1
  • S. K. Gupta
    • 1
  • B. S. Dunbar
    • 2
  1. 1.Department of ImmunopathologyPostgraduate Institute of Medical Education and ResearchChandigarh(India)
  2. 2.Department of Cell BiologyBaylor College of MedicineHoustonUSA

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