, Volume 45, Issue 8, pp 742–747 | Cite as

Gradient distribution pattern of muscarinic receptors in N1E 115 mouse neuroblastoma cells

  • N. H. Fraeyman
  • M. -A. Buyse
Research Articles


The distribution pattern of muscarinic receptors in N1E 115 mouse neuroblastoma cells after linear and non-linear gradient centrifugation was investigated. In untreated cells, at least two forms of the receptors, with different densities, were found.

Key words

Muscarinic receptors N1E 115 neuroblastoma cells gradient distribution concanavalin A 


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  1. 1.
    Stadel, M. M., Strulovici, B., Nambi, P., Lavin, T. N., Briggs, M. M., Caron, M. G., and Lefkowitz, R. J., J. biol. Chem.258 (1983) 3032.PubMedGoogle Scholar
  2. 2.
    Strader, C. D., Sibley, D. R., and Lefkowitz, R. J., Life Sci.35 (1984) 1601.CrossRefPubMedGoogle Scholar
  3. 3.
    Waldo, G. L., Northup, J. K., Perkins, J. P., and Harden, T. K., J. biol. Chem.258 (1983) 13900.PubMedGoogle Scholar
  4. 4.
    Toews, M. L., Waldo, G. L., Harden, T. K., and Perkins, J. P., J. biol. Chem.259 (1984) 11844.PubMedGoogle Scholar
  5. 5.
    Clark, R. B., Friedman, J., Prashad, N., and Ruoho, A. E., J. cycl. Nucl. Res.10 (1985) 97.Google Scholar
  6. 6.
    Kassis, S., and Sullivan, M., J. cycl. Nucl. Res.11 (1986) 35.Google Scholar
  7. 7.
    Harden, T. K., Petch, L. A., Traynelis, S. F., and Waldo, G. L., J. biol. Chem.260 (1985) 13060.PubMedGoogle Scholar
  8. 8.
    McKinney, M., and Richelson, E., A. Rev. Pharmac. Toxic.24 (1984) 121.CrossRefGoogle Scholar
  9. 9.
    Fraeyman, N., and Buyse, M.-A., Fund. clin. Pharmac. (1989) submitted.Google Scholar
  10. 10.
    Buyse, M.-A., Lefebvre R., and Fraeyman, N., Tissue Cell (1989) submitted.Google Scholar
  11. 11.
    Kind, P. R., and King E. J., J. clin. Path.7 (1954) 322.PubMedGoogle Scholar
  12. 12.
    Bradford, M. M., Analyt. Biochem.72 (1976) 248.PubMedGoogle Scholar
  13. 13.
    Scarborough, G. A., J. biol. Chem250 (1975) 1106.PubMedGoogle Scholar
  14. 14.
    Lutton, J. K. Frederich, R. C., and Perkins, J. P., J. biol. Chem.Google Scholar
  15. 15.
    Wallach, D. F. H., in: Membrane Molecular Biology of Neoplastic cells, p. 435. Ed. D. F. H. Wallach. Elsevier Scientific Publ., Amsterdam 1975.Google Scholar
  16. 16.
    Lee, J.-H., and El-Fakahany, E. E., Eur. J. Pharmac.110 (1985) 263.CrossRefGoogle Scholar
  17. 17.
    Scott, R. E., Science194 (1976) 743.PubMedGoogle Scholar
  18. 18.
    Rademaker, B., Kramer, K., Stroes, J. W., Vlug, J., Krielaart, M., and Zaagsma, J., Eur. J. Pharmac.111 (1985) 31.CrossRefGoogle Scholar

Copyright information

© Birkhäuser Verlag 1989

Authors and Affiliations

  • N. H. Fraeyman
    • 1
  • M. -A. Buyse
    • 1
  1. 1.Heymans Institute of PharmacologyUniversity of Ghent Medical SchoolGhent(Belgium)

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