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Helicobacter pylori in a costa rican dyspeptic patient population

  • B. Morera-Brenes
  • R. Sierra
  • R. Barrantes
  • J. Jonasson
  • C. E. Nord
Notes

Abstract

Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination.Helicobacter pylori DNA was detected by PCR in 60 (92 %) of the patients, andHelicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98 % (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material.

Keywords

Polymerase Chain Reaction Internal Medicine Patient Population Histological Examination Bacterial Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • B. Morera-Brenes
    • 1
    • 2
  • R. Sierra
    • 1
  • R. Barrantes
    • 1
  • J. Jonasson
    • 2
  • C. E. Nord
    • 2
  1. 1.Instituto de Investigaciones en SaludUniversidad de Costa RicaSan JoséCosta Rica
  2. 2.Department of Microbiology, Karolinska InstituteHuddinge University HospitalHuddingeSweden

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