Helicobacter pylori in a costa rican dyspeptic patient population

  • B. Morera-Brenes
  • R. Sierra
  • R. Barrantes
  • J. Jonasson
  • C. E. Nord


Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination.Helicobacter pylori DNA was detected by PCR in 60 (92 %) of the patients, andHelicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98 % (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material.


Polymerase Chain Reaction Internal Medicine Patient Population Histological Examination Bacterial Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Graham DY Helicobacter pylori: its epidemiology and its role in duodenal ulcer disease. Journal of Gastroenterology and Hepatology 1991, 6: 97–105.Google Scholar
  2. 2.
    Morgan DR, Leunk RD Pathogenesis of infection byCampylobacter pylori. In: Blaser MJ (ed):Campylobacter pylori in gastritis and peptic ulcer disease. Igaku-Shoin, New York, 1989, p. 115–133.Google Scholar
  3. 3.
    Parsonnet J, Friedman GD, Vandersteen DP, Chang Y, Volgeman JH, Orentreinch N, Sibley RK Helicobacter pylori infection and the risk of gastric carcinoma. New England Journal of Medicine 1991, 325: 1127–1131.PubMedGoogle Scholar
  4. 4.
    Hoshina S, Kahn SM, Jiang W, Green PH, Neu HC, Chin N, Morotomi M, LoGerfo P, Weinstein IB Direct detection and amplification ofHelicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies. Diagnostic Microbiology and Infectious Diseases 1990, 13: 473–479.CrossRefGoogle Scholar
  5. 5.
    Nguyen A-M H, Engstrand L, Genta RM, Graham DY, El-Zaatari FAK Detection ofHelicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction. Journal of Clinical Microbiology 1993, 31: 783–787.PubMedGoogle Scholar
  6. 6.
    Ho S-A, Hoyle JA, Lewis FA, Secker AD, Cross D, Mapstone NP, Dixon MF, Wyatt JI, Tompkins DS, Taylor GR, Quirke P Direct polymerase chain reaction test for detection ofHelicobacter pylori in humans and animals. Journal of Clinical Microbiology 1991, 29: 2543–2549.PubMedGoogle Scholar
  7. 7.
    Clayton CL, Kleanthous H, Coates PJ, Morgan DD, Tabaqchali S Sensitive detection ofHelicobacter pylori by using polymerase chain reaction. Journal of Clinical Microbiology 1992, 30: 192–200.PubMedGoogle Scholar
  8. 8.
    Hammar M, Tyszkiewicz T, Wadström T, O'Toole PW Rapid detection ofHelicobacter pylori in gastric biopsy material by polymerase chain reaction. Journal of Clinical Microbiology 1992, 30: 54–58.PubMedGoogle Scholar
  9. 9.
    Valentine JL, Arthur RR, Mobley HLT, Dick JD Detection ofHelicobacter pylori by using the polymerase chain reaction. Journal of Clinical Microbiology 1991, 29: 689–695.PubMedGoogle Scholar
  10. 10.
    Jackson DP, Lewis FA, Taylor GR, Boylston AW, Quirke P Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. Journal of Clinical Pathology 1990, 43: 499–504.PubMedGoogle Scholar
  11. 11.
    Shibata DK, Arnheim N, Martin WJ Detection of human papiloma virus in paraffin-embedded tissue using the polymerase chain reaction. Journal of Experimental Medicine 1988, 167: 225–230.CrossRefPubMedGoogle Scholar
  12. 12.
    Westblom TU, Phadnis S, Yang P, Czinn SJ Diagnosis ofHelicobacter pylori infection by means of a polymerase chain reaction assay for gastric juice aspirates. Clinical Infectious Diseases 1993, 16: 367–371.PubMedGoogle Scholar
  13. 13.
    Mapstone NP, Lynch DAF, Lewis FA, Axon ATR, Tompkins DS, Dixon MF, Quirke P PCR identification ofHelicobacter pylori in faeces from gastritis patients. Lancet 1993, 341: 447.CrossRefGoogle Scholar
  14. 14.
    Barrantes E, Durán S, de Mezerville L Campylobacter pylori: aislamiento en mucosa gástrica. Revista Costarricense de Ciencias Médicas 1989, 10: 31–40.Google Scholar
  15. 15.
    Dehesa M, Dooley CP, Cohen H, Fitzgibbons PL, Perez-Perez GI, Blaser MJ High prevalence ofHelicobacter pylori infection and histologic gastritis in asymptomatic Hispanics. Journal of Clinical Microbiology 1991, 29: 1128–1131.PubMedGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • B. Morera-Brenes
    • 1
    • 2
  • R. Sierra
    • 1
  • R. Barrantes
    • 1
  • J. Jonasson
    • 2
  • C. E. Nord
    • 2
  1. 1.Instituto de Investigaciones en SaludUniversidad de Costa RicaSan JoséCosta Rica
  2. 2.Department of Microbiology, Karolinska InstituteHuddinge University HospitalHuddingeSweden

Personalised recommendations