Routine laboratory diagnosis of continuous ambulatory peritoneal dialysis peritonitis using centrifugation/lysis and saponin-containing media

  • P. C. Taylor
Notes

Abstract

This study examines the application in a routine laboratory of a method for concentrating and lysing leucocytes in continuous ambulatory peritoneal dialysis (CAPD) effluent to diagnose peritonitis. Between November 1986 and April 1990 pathogens from 42 of 82 (51 %) CAPD specimens from patients with acute peritonitis failed to grow on horse blood agar (HBA) after direct inoculation. Recovery rates of pathogens were 74 % (6 of 82) from a solid medium containing saponin and 89 % (73 of 82) after lysing the leucocytes before inoculating HBA. Microbial sequestration in leucocytes was demonstrated in 73% (60 of 82) of specimens, while pathogens from 11 % (9 of 82) were grown only from a liquid medium. Thirteen of 17 specimens with<500 leucocytes/mm3 grew on all three solid media. Gram stain microscopy revealed organisms in 51 % (41 of 81) of specimens. The method, using apparatus and media prepared for everyday examination of specimens, is sensitive, specific and yields single colonies of most species within 24 h.

Keywords

Internal Medicine Agar Liquid Medium Peritonitis Peritoneal Dialysis 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • P. C. Taylor
    • 1
  1. 1.Department of MicrobiologyThe Prince of Wales HospitalRandwickAustralia

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