Abstract
Somatic hybridization, a process of combining protoplasts from different plants, can provide new sources of disease resistances for plants. In the case of wild and cultivatedSolanum species, the hybrids express resistances from each partner in the fusion and can often be crossed with cultivars to improve agronomic characteristics of the tubers. Restriction fragment length polymorphism (RFLP) analyses can provide a means for determining that the plants being investigated are actually hybrids as well as a means for following the introgression of DNA into progeny lines. These points are addressed in this paper with specific reference to somatic hybrids betweenSolanum brevidens and potato.
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Austin, S., Baer, M. A. & Helgeson, J.P., 1985. Transfer of resistance to potato leaf roll virus fromSolanum brevidens intoSolanum tuberosum by somatic fusion. Plant Science 39: 75–82.
Austin, S., Lojkowska, E., Elenfeldt, M., Kelman, A. & Helgeson, J.P., 1988. Fertile interspecific somatic hybrids ofSolanum: A novel source of resistance to Erwinia soft rot. Phytopathology 78: 1216–1220.
Bonierbale, M.W., Plasted, R.L. & Tanksley, S.D., 1988. RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120: 1095–1103.
Ehlenfeldt, M.K. & Helgeson, J.P., 1987. Fertility of somatic hybrids from protoplast fusions ofSolanum brevidens andS. tuberosum. Theoretical and Applied Genetics 73: 395–402.
Haberlach, G.T., Cohen, B., Reichert, N., Baer, M., Towil, L. & Helgeson, J.P., 1985. Isolation, culture and regeneration of protoplasts from potato and several relatedSolanum species. Plant Science 39: 67–74.
Hanneman, R.E., Jr. & Bamberg, J., 1986. Inventory of tuber-bearingSolanum species. Bulletin 533, Wisconsin Agricultural Experimental Station. pp. 216.
Helgeson, J.P., Hunt, G.T., Haberlach, G.T. & Austin, S., 1986. Somatic hybrids betweenSolanum brevidens andSolanum tuberosum: Expression of a late blight resistance gene and potato leaf roll resistance. Plant Cell Reports 3: 212–214.
Hunt, G. & Helgeson, J.P., 1989. A medium and simplified procedure for growing single cells fromSolanum species. Plant Science 60: 251–257.
Williams, C.E., Hunt, G. & Helgeson, J.P., 1990. Fertile somatic hybrids ofSolanum species: RFLP analysis of a hybrid and its sexual progeny from crosses with potato. Theoretical and Applied Genetics 80: 545–551.
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Helgeson, J.P. New genes for disease resistances through somatic hybridization. Netherlands Journal of Plant Pathology 98 (Suppl 2), 223–229 (1992). https://doi.org/10.1007/BF01974489
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DOI: https://doi.org/10.1007/BF01974489