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Netherlands Journal of Plant Pathology

, Volume 86, Issue 2, pp 69–78 | Cite as

Lonicera latent virus, a new carlavirus serologically related to poplar mosaic virus: some properties and inactivation in vivo by heat treatment

  • F. A. Van Der Meer
  • D. Z. Maat
  • J. Vink
Article

Abstract

A virus with elongate particles (656 nm) was isolated from severalLonicera species. This virus, apparently belonging to the carlavirus group, is serologically distantly related to shallot latent virus and closely related to poplar mosaic virus. The inability to infect poplar and two other hosts of poplar mosaic virus characterizes the virus fromLonicera as a new virus which was namedLonicera latent virus.

The virus was easily sap-transmissible but was not transmitted byMyzus persicae.

Dilution end-point was about 10−3, thermal inactivation between 65°C and 80°C and ageing in vitro 1–6 days.

Heat treatment, combined with tip-rooting appeared to be a good method to eliminate the virus from severalLonicera species and cultivars.

Samenvatting

In verschillende soorten en cultivars van het geslachtLonicera (kamperfoelie) blijkt een virus voor te komen dat gemakkelijk door sapinoculatie kan worden overgebracht op kruidachtige planten.

Een tegen gezuiverd virus bereid antiserum had een titer van ca. 4096. Er kon mee worden aangetoond dat het virus van kamperfoelie serologisch nauw verwant is met populieremozaïekvirus (Tabel 1). Het virus van kamperfoelie is echter niet in staat om populier,Phaseolus vulgaris ‘Bataaf’ enVigna sinensis te infecteren en wordt mede daarom als een afzonderlijk virus beschouwd. Het wordt aangeduid als latent kamperfoelievirus (Lonicera latent virus) en behoort evenals populieremozaïekvirus tot de carlavirusgroep (aardappelvirus-S-groep).

Het virus blijkt vrij gemakkelijk te kunnen worden geëlimineerd door besmette kamperfoelieplanten gedurende ongeveer zes weken een warmtebehandeling (37°C) te geven en daarna de uiterste toppen (1 cm) te stekken. Van verschillende cultivars werd op deze wijze virusvrij uitgangsmateriaal verkregen.

Additional keywords

Nicotiana megalosiphon virus adaptation yellow vein mosaic ofLonicera japonica 

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1980

Authors and Affiliations

  • F. A. Van Der Meer
    • 1
  • D. Z. Maat
    • 1
  • J. Vink
    • 1
  1. 1.Instituut voor Plantenziektenkundig OnderzoekWageningenthe Netherlands

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