Netherlands Journal of Plant Pathology

, Volume 95, Supplement 1, pp 175–185 | Cite as

Effect of interrupted leaf wetness periods on pustule development of Puccinia recondita f. sp. tritici on wheat

  • R. E. Stuckey
  • J. C. Zadoks


In growth chamber experiments, seedlings of a susceptible wheat cultivar (Rubis) were inoculated with urediospores of wheat leaf rust. Inoculated seedlings were incubated in a moist chamber. The period of exposure to leaf wetness varied in duration and progressed with or without one or more interruptions of variable duration. During the interruptions the leaves with germlings were dry. At near-optimal temperature, leaf wetness periods of six h resulted in 60 to 65% of the pustules produced with 12 or 24 h wetness periods. Interruption of a 6 h leaf wetness period by a 1 h dry period was most damaging to the rust germlings at about three to four h after inoculation. The damage became visible as a reduction in the number of resulting pustules and as a prolongation of the median latency period. Epidemiological implications of leaf wetness interruptions are discussed.


Kiemplanten van het vatbare tarweras Rubis werden geïnoculeerd met uredosporen van de bruine roest van tarwe. De proeven vonden plaats in klimaatkamers. Geïnoculeerde planten werden geïncubeerd in incubatiehokjes met een zeer hoge luchtvochtigheid, teneinde condensatiedruppels op de bladeren en aldus bladnatperioden te krijgen. De toegediende bladnatperioden varieerden in tijdsduur en zij verliepen met of zonder één of meer onderbrekingen, ook weer van variërende duur. Tijdens de onderbrekingen stonden de planten met roestkiemlingen in het licht; het blad was dan droog. Bij ongeveer optimale temperatuur en bladnatperioden van 6 uur was het aantal verkregen puistjes 60 à 65% van het aantal verkregen puistjes na een bladnatperiode van 12 of 24 uur. Onderbreking van de 6-urige bladnatperiode door een 1-urige droge periode was voor de roestkiemlingen het schadelijkst ongeveer 3 tot 4 uur na inoculatie. De schade kwam tot uitdrukking in het kleinere aantal geproduceerde puistjes en in een verlenging van de mediane latentieperiode. De epidemiologische betekenis van onderbrekingen van de bladnatperiode wordt besproken.

Additional keywords

germination latency period pustulation uredinia urediniospores 


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  1. Mehta, Y. & Zadoks, J.C., 1970. Uredospore production and sporulation period ofPuccinia recondita f. sp.triticina on primary leaves of wheat. Netherlands Journal of Plant Pathology 76: 267–276.Google Scholar
  2. Mehta, Y. & Zadoks, J.C., 1971. Note on the efficiency of a miniaturized cyclone spore collector. Netherlands Journal of Plant Pathology 77: 60–63.Google Scholar
  3. Shearer, B.L. & Zadoks, J.C., 1972. The latent period ofSeptoria nodorum in wheat. I. The effect of temperature and moisture treatments under controlled conditions. Netherlands Journal of Plant Pathology 78: 231–241.Google Scholar
  4. Spain, J.D., 1982. BASIC microcomputer models in biology. Reading (Mass.), Addison-Wesley. 354 pp.Google Scholar
  5. Teng, P.S. & Close, R.C., 1978. The effect of temperature and uredinium density on urediniospore production, latent period and infectious period ofPuccinia hordei Otth. New Zealand Journal of Agricultural Research 21: 287–296.Google Scholar
  6. Van Hees-Boukema, E.M. & Zadoks, J.C., 1986. Postponed germination ofPuccinia recondita urediospores deposited on wheat seedlings. II. Infectivity ofPuccinia recondita urediospores deposited on wheat leaves. Netherlands Journal of Plant Pathology 92: 71–80.Google Scholar
  7. Wiese, M.V., 1981. Compendium of Wheat Diseases. St. Paul, American Phytopathological Society. 106 pp.Google Scholar
  8. Zadoks, J.C., Chang, T.T. & Konzak, C.F., 1974. A decimal code for the growth stages of cereals. Weed Research 14: 415–421.Google Scholar
  9. Zadoks, J.C. & Groenewegen, L.J.M., 1967. On light-sensitivity in germinating uredospores of wheat brown rust. Netherlands Journal of Plant Pathology 73: 83–102.Google Scholar
  10. Zadoks, J.C. & Van Hees-Boukema, E.M., 1986. Postponed germination ofPuccinia recondita urediospores deposited on wheat seedlings. I. Ripening and longevity of urediospores with postponed germination. Netherlands Journal of Plant Pathology 92: 57–69.Google Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1989

Authors and Affiliations

  • R. E. Stuckey
    • 1
  • J. C. Zadoks
    • 2
  1. 1.Laboratory of PhytopathologyUniversity of KentuckyLexingtonUSA
  2. 2.Department of Plant PathologyWageningen Agricultural UniversityWageningenthe Netherlands

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