Netherlands Journal of Plant Pathology

, Volume 99, Issue 3, pp 105–113 | Cite as

Regulators of ethylene biosynthesis or activity as a tool for reducing susceptibility of host plant tissues to infection by Botrytis cinerea

Articles

Abstract

Several compounds were tested for their ability to reduce development of grey mould on rose, tomato, pepper, eggplant, French bean andSenecio sp. Removal of ethylene from the atmosphere surrounding rose flowers, or leaves of tomato and pepper, by potassium permanganate, resulted in slower grey mould development. Inhibition of ethylene activity by 2,5-norbornadiene controlled disease on all crops but tomato. Carbon dioxide controlled grey mould on roses, but the potential for use of these agents is in doubt. Inhibitors of ethylene biosynthesis such as aminooxyacetic acid (AOA), cobalt ion, the uncoupler 2,4-dinitrophenol and the radical scavenger salicylic acid were differentially effective in controlling the disease in the various hosts. Fifty mM AOA reduced grey mould on rose flowers by up to 97% when flowers were partially aerated. AOA was not phytotoxic on the tested rose cvs Golden Times and Jaguar. Combinations of ethylene absorption, inhibition of ethylene activity and ethylene biosynthesis did not result in better control as compared with the disease reduction ability of the compounds alone, tested on the various hosts. Application of benzyladenine, which reduces the host responsiveness to ethylene, resulted in 39–99% grey mould reduction in rose flowers and in leaves of tomato andSenecio sp. but was not effective on pepper or eggplant. Manipulation of ethylene presence and of host plant susceptibility to grey mould is discussed.

Additional keywords

aminooxyacetic acid benzyladenine carbon dioxide cobalt 2,4-dinitrophenol eggplant French bean 2,5-norbornadiene potassium permanganate pepper (radical scavenger) rose salicylic acid Senecio tomato (uncoupler) 

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References

  1. Aharoni, N., Liberman, M. & Sisler, H., 1979. Patterns of ethylene production in senescing leaves. Plant Pathology 64: 796–800.Google Scholar
  2. Apelbaum, A., Wang, S.Y., Burgoon, A.C., Baker, J.G. & Liberman, M., 1981. Inhibition of the conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by structural analogs, inhibitors of electron transfer, uncouplers of oxidative phosphorylation and free radical scavengers. Plant Physiology 67: 74–79.Google Scholar
  3. Archer, S.A. & Hislop, E.D., 1975. Ethylene in host pathogen relationships. Annals of Applied Biology 81: 121–126.Google Scholar
  4. Barkai-Golan, R., Lavy-Meir, G. & Kopeliovitch, E. 1989. Effects of ethylene on the susceptibility toBotrytis cinerea infection of different tomato genotypes. Annals of Applied Biology 116: 391–396.Google Scholar
  5. Barmore, C.R., Wheaton, T.A. & McConrnack, A.A., 1976. Ethylene degreening of Bears' lemons. HortScience 11: 588–590.Google Scholar
  6. Biles, C.L., Abeles, F.B. & Wilson, C.L., 1990. The role of ethylene in anthracnose of cucumber,Cucumis sativus, caused byColletotrichum lagenarium. Phytopathology 80: 732–736.Google Scholar
  7. Boller, T., 1982. Ethylene induced biochemical defenses against pathogens. In: Waring P.F., (Ed.), Plant growth substance. Academic Press, New York. p. 302–312.Google Scholar
  8. Burg, S.P. & Burg, E.A., 1967. Molecular requirements for the biological activity of ethylene. Plant Physiology 42: 144–152.Google Scholar
  9. Dehne, H.-W., Blankenagel, R. & Schönbeck, F., 1981. Influence of ethylene-releasing substances on the occurrence ofHelminthosporium sativum on winter barley and on the yield under practical conditions. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 88: 206–209.Google Scholar
  10. Elad, Y., 1988a. Involvement of ethylene in the disease caused byBotrytis cinerea on rose and carnation flowers and the possibility to control. Annals of Applied Biology 113: 589–598.Google Scholar
  11. Elad, Y., 1988b. Latent infection ofBotrytis cinerea. in rose flowers and combined chemical physiological control of the disease. Crop Protection 7: 361–366.CrossRefGoogle Scholar
  12. Elad, Y., 1989. Effect of abiotic conditions on development of gray mold of rose and scanning electron microscopy. Phytopathologia Mediterranea 28: 122–130.Google Scholar
  13. Elad, Y., 1990. Production of ethylene by tissues of tomato, pepper, French-bean and cucumber in response to infection byBotrytis cinerea. Physiological and Molecular Plant Pathology 36: 277–287.CrossRefGoogle Scholar
  14. Elad, Y. & Volpin, H., 1988. The involvement of ethylene and calcium in grey mold of pelargonium, ruscus and rose flowers. Phytoparasitica 16: 119–131.Google Scholar
  15. Goodman, R.N., Kiraly, Z. & Wood, K.R., 1986. The biochemistry and physiology of plant disease. University of Missouri Press, Columbia 433 pp.Google Scholar
  16. Granger, R.L. & Rousselle, G.L., 1984. Effect of ethylene removal by alumina/potassium permanganate on MacIntosh apples in regular and controlled atmosphere storages. Acta Horticulturae 157: 57–150.Google Scholar
  17. Halevy, A.H. & Kofranek, A.M., 1977. Silver treatment of carnation flowers for reducing ethylene damage and extending longevity. Journal of the American Society of Horticultural Sciences 102: 76–77.Google Scholar
  18. Leslie, G.A. & Romani, R.J., 1986. Salicylic acid: A new inhibitor of ethylene biosynthesis. Plant Cell Reports 5:144–146. Manning, K., 1985. The ethylene forming enzyme system in carnation flowers. In: Roberts, J.A. and Tucker, G.A. (Eds) Ethylene and plant development. Butterworths, London, p. 63–92.Google Scholar
  19. McNicol, R.J., Williamson, B. & Young, K., 1989. Ethylene production by black currant flowers infected byBotrytis cinerea. Acta Horticulturae. 226: 209–215.Google Scholar
  20. Phillips, D.J., Margosan, D.A. & Fouse, D.C. 1985. Postharvest control of Botrytis rot of roses with carbon dioxide. Plant Disease 69: 789–790.Google Scholar
  21. Philosoph-Hadas, S., Pesis, E., Meir, S., Reuveni, A. & Aharoni, N., 1989. Ethylene-enhanced senescence of leafy vegetables and fresh herbs. Acta Horticulturae 258: 37–42.Google Scholar
  22. Saltveit, M.E., Jr. 1980. An inexpensive chemical scrubber for oxidizing volatile organic contaminants in gases and storage room atmosphere. HortScience 15: 759–760.Google Scholar
  23. Sisler, E.C., 1979. Measurement of ethylene binding in plant tissue. Plant Physiology 64: 538–542.Google Scholar
  24. Sisler, E.C., Goren, R. & Huberman, M., 1985. Effect of 2,5-norbornadiene on abscission and ethylene production in citrus leaf explants. Physiologia Plantarum 63: 114–120.Google Scholar
  25. Smith, A.R., Evans, D.E., Smith, P.G. & Hall, M.A., 1985. Ethylene metabolism inPisum sativum L andVicia faba L. In: Roberts, J.A. & Tuckere, G.A. (Eds), Ethylene and plant development. Butterworths, London, p. 139–145.Google Scholar
  26. Smith, A.R. & Hall, M.A., 1985. Ethylene binding. In: Roberts, J.A. & Tucker, (Eds), Ethylene and plant development. Butterworths, London, p. 101–116.Google Scholar
  27. Staby, G.L., Cunningham, M.S., Holstead, C.L., Kelly, J.W., Konjoian, P.S., Eisenberg, B.A. & Dressler, B.S., 1984. Storage of rose and carnation flowers. Journal of the American Society of Horticultural Sciences 109: 193–197.Google Scholar
  28. Veen, H., 1987. Use of inhibitors of ethylene action. Acta Horticulturae 201: 213–222.Google Scholar
  29. Yang, S.F. & Hoffman, N.E., 1984. Ethylene biosynthesis and its regulation in higher plants. Annual Review of Plant Physiology 35: 155–189.CrossRefGoogle Scholar
  30. Yu, Y.B., Adams, D.O. & Yang, S.F., 1979. I-Aminocyclopropane-carboxylate synthase, a key enzyme in ethylene biosynthesis. Archives of Biochemistry and Biophysics 198: 280–286.CrossRefPubMedGoogle Scholar
  31. Yu, Y., Adams, D.O. & Yang, S.F., 1980. Inhibition of ethylene production by 2,4-dinitrophenol and high temperature. Plant Physiology 66: 286–290.Google Scholar
  32. Yu, Y. & Yang, S.F., 1979. Auxin-induced ethylene production and its inhibition by aminooxyvinylgycine and cobalt ion. Plant Physiology 64: 1074–1077.Google Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 1993

Authors and Affiliations

  • Y. Elad
    • 1
  1. 1.Department of Plant PathologyAgricultural Research Organization, The volcani CenterBel DaganIsrael

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