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Transgenic Research

, Volume 3, Issue 2, pp 127–133 | Cite as

Regulation of insulin-like growth factor-l and binding protein-3 expression in oMtla-oGH transgenic mice

  • Jesse C. Chow
  • James D. Murray
  • Daniel Pomp
  • Ransom L. Baldwin
  • Christopher C. Calvert
  • Anita M. Oberbauer
Papers

Abstract

Growth hormone (GH)-transgenic mice provide a model for studying hormonal regulation of gene products responsible for efficient lean growth. Insulin-like growth factor-I (IGF-I) and IGF binding protein-3 (BP-3) are two products involved in mediating the growth promoting actions of GH. Mice carrying the ovine metallothionein la-ovine growth hormone (oMtla-oGH) transgene were used to study GH regulation of IGF-I and PB-3 expression because these mice do not exhibit elevated basal oGH levels without transgene stimulation by exogenous zinc. C57B1/6XCBA mice with (transgenic=TG) and without (control=C) the oMtla-oGH transgene were activated (+Zn) or inactivated (-Zn) by the addition or removal of 25 mM zinc sulfate in the drinking water. Plasma IGF-I and BP-3 levels were determined by radioimmunoassay and western ligand blotting, respectively. Hepatic IGF-I and BP-3 mRNA levels were determined by slot-blot analysis. TG+Zn mice had higher plasma IGF-I (p<0.05) and hepatic IGF-I mRNA (p<0.05) levels as compared to TG-Zn, C+Zn and C-Zn mice. Plasma IGF-I and hepatic IGF-I mRNA levels in TG-Zn mice were not different from C+Zn and C-Zn mice. Removal of Zn decreased hepatic IGF-I mRNA levels to C levels in TG mice. Plasma BP-3 and hepatic BP-3 mRNA levels in TG+Zn mice were increased (p<0.05) as compared to TG-Zn, C-Zn and C+Zn. Plasma BP-3 and hepatic BP-3 mRNA levels did not differ between TG-Zn, C-Zn and C+Zn mice. Expression of the transgene also increased the level of plasma BP-3 during pregnancy as compared to that observed for pregnant C mice. We conclude that oGH regulates IGF-I and BP-3 expression in the oMtla-oGH transgenic mouse model system.

Keywords

IGF-I BP-3 GH transgenic mouse pregnancy 

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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Jesse C. Chow
    • 1
  • James D. Murray
    • 1
    • 2
  • Daniel Pomp
    • 1
    • 3
  • Ransom L. Baldwin
    • 1
  • Christopher C. Calvert
    • 1
  • Anita M. Oberbauer
    • 1
  1. 1.Departments of Animal Science, School of Veterinary MedicineUniversity of California-DavisDavisUSA
  2. 2.Department of Reproduction, School of Veterinary MedicineUniversity of California-DavisDavisUSA
  3. 3.Oklahoma State UniversityStillwaterUSA

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