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Transgenic Research

, Volume 3, Issue 3, pp 182–194 | Cite as

Reporter genes in transgenic mice

  • Cunqi Cui
  • Maqsood A. Wani
  • David Wight
  • John Kopchick
  • Peter J. Stambrook
Review

Abstract

Althoughin vivo models utilizing endogenous reporter genes have been exploited for many years, the use of reporter transgenes to dissect biological issues in transgenic animals has been a relatively recent development. These transgenes are often, but not always, of prokaryotic origin and encode products not normally associated with eukaryotic cells and tissues. Some encode enzymes whose activities are detected in cell and tissue homogenates, whereas others encode products that can be detectedin situ at the single cell level. Reporter genes have been used to identify regulatory elements that are important for tissue-specific gene expression or for development; they have been used to producein vivo models of cancer; they have been employed for the study ofin vivo mutagenesis; and they have been used as a tool in lineage analysis and for marking cells in transplanation experiments. The most commonly usedin situ reporter gene islacZ, which encodes a bacterial β-galactosidase, a sensitive histochemical marker. Although it has been used with striking success in cultured cells and in transgenic mouse embryos, its postnatalin vivo expression has been unreliable and disappointing. Nevertheless, the ability to express reporter genes in transgenic mice has been an invaluable resource, providing insights intoin vivo biological mechanisms. The development of newin vivo models, such as those in which expression of transgenes can be activated or repressed, should produce transgenic animal systems that extend our capacity to address heretofore unresolved biological questions.

Keywords

Transgenic Mouse Reporter Gene Transgenic Animal Lineage Analysis Single Cell Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Cunqi Cui
    • 1
  • Maqsood A. Wani
    • 1
  • David Wight
    • 2
  • John Kopchick
    • 2
  • Peter J. Stambrook
    • 1
  1. 1.Department of Anatomy and Cell BiologyUniversity of CincinnatiCincinnatiUSA
  2. 2.Edison InstituteOhio UniversityAthensUSA

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