Agents and Actions

, Volume 14, Issue 3–4, pp 488–493 | Cite as

Histamine release from the cultured mouse mast cell line PT 18 in response to immunologic and non-immunologic stimuli

  • K. E. Barrett
  • D. H. Pluznik
  • D. D. Metcalfe
Histamine Release and Pathological States


Cultured mouse mast cells that are dependent on spleen-derived factors for their proliferation and maintenance have been shown rapidly to release histamine in response to immunologic stimuli, concanavalin A, and ionophores A23187 and Br-x537A. These cells did not, however, release histamine when treated with compound 48/80 or adenosine 5′-triphosphate. Immunologically mediated histamine release was inhibited by theophylline and quercetin, but not by disodium cromoglycate. The results presented here are discussed in terms of the possible relationship of this cell line to mucosal mast cells.


Adenosine Mast Cell Histamine Quercetin Theophylline 
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Copyright information

© Birkhäuser Verlag 1984

Authors and Affiliations

  • K. E. Barrett
    • 1
  • D. H. Pluznik
    • 2
  • D. D. Metcalfe
    • 1
  1. 1.Allergic Diseases Section, Laboratory of Clinical InvestigationNational Institute of Allergy and Infectious DiseasesBethesdaUSA
  2. 2.Laboratory of Microbiology and Immunology, National Institute of Dental ResearchNational Institutes of HealthBethesdaUSA

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