Advertisement

Agents and Actions

, Volume 14, Issue 3–4, pp 488–493 | Cite as

Histamine release from the cultured mouse mast cell line PT 18 in response to immunologic and non-immunologic stimuli

  • K. E. Barrett
  • D. H. Pluznik
  • D. D. Metcalfe
Histamine Release and Pathological States

Abstract

Cultured mouse mast cells that are dependent on spleen-derived factors for their proliferation and maintenance have been shown rapidly to release histamine in response to immunologic stimuli, concanavalin A, and ionophores A23187 and Br-x537A. These cells did not, however, release histamine when treated with compound 48/80 or adenosine 5′-triphosphate. Immunologically mediated histamine release was inhibited by theophylline and quercetin, but not by disodium cromoglycate. The results presented here are discussed in terms of the possible relationship of this cell line to mucosal mast cells.

Keywords

Adenosine Mast Cell Histamine Quercetin Theophylline 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    E. Razin, R.L. Stevens, F. Akiyama, K. Schmid andK.F. Austen,Culture from mouse bone marrow of a subclass of mast cells possessing a distinct chondroitin sulfate proteoglycan with glycosaminoglycans rich in N-acetylgalactosamine-4,6-disulfate, J. biol. Chem.257, 7229–7236 (1982).PubMedGoogle Scholar
  2. [2]
    J.W. Schrader,The in vitro production and cloning of the P-cell, a bone marrow-derived null cell that expresses H-2 and Ia-antigens, has mast cell-like granules, and is regulated by a factor released by activated T cells, J. Immun.126, 452–458 (1981).PubMedGoogle Scholar
  3. [3]
    D.H. Pluznik, N.S. Tare, M.M. Zatz andA.L. Goldstein,A mast/basophil cell line dependent on colony stimulating factor, Exp. Hematol.10, 211–218 (1982).Google Scholar
  4. [4]
    B. Sredni, M.M. Friedman, C.E., Bland andD.D., Metcalfe,Ultrastructural, biochemical and functional characteristics of histamine-containing cells cloned from mouse bone marrow: tentative identification as mucosal mast cells, J. Immun.131, 915–922 (1983).PubMedGoogle Scholar
  5. [5]
    C.E. Bland, K.L. Rosenthal, D.H. Pluznik C. Riedel and andD.D. Metcalfe,Comparison of glycosaminoglycans synthesized by cultured mast cells and granulated lymphocytes, Fedn Proc.42, 444 (1983).Google Scholar
  6. [6]
    J.W. Schrader andI. Clark-Lewis,T cell hybridoma-derived regulatory factors I. Production of T cell growth factor following stimulation by concanavalin A, J. Immun.126, 1101–1105 (1981).PubMedGoogle Scholar
  7. [7]
    K.E. Barrett, M. Ennis, andF.L. Pearce,Mast cells isolated from guinea pig lung: characterization and studies on histamine secretion, Agents and Actions13, 122–126 (1983).PubMedGoogle Scholar
  8. [8]
    G. Atkinson, M. Ennis andF.L., Pearce,The effect of alkaline earth cations on the release of histamine from rat peritoneal, mast cells treated with compound 48/80 and peptide 401 Br. J. Pharmac.65, 395–402 (1979).Google Scholar
  9. [9]
    F.L. Pearce, K.E. Barrett andJ.R. White,Histamine secretion from mast cells treated with chlortetracycline (aureomycin): a novel calcium ionophore, Agents and Actions13, 117–122 (1983).PubMedGoogle Scholar
  10. [10]
    K.E. Barrett andF.L. Pearce,A comparison of histamine secretion from peritoneal mast cells of the mouse and the rat, Int. Archs Allergy appl. Immun.72, 234–238 (1983).Google Scholar
  11. [11]
    P.A. Shore, A. Burkhalter andV.H. Cohn,A method for the fluorimetric assay of histamine in tissues, J. Pharmac. exp. Ther.127, 182–186 (1959).Google Scholar
  12. [12]
    F.L. Pearce, A.D. Befus, J. Gauldie andJ. Bienenstock,Mucosal mast cell. II. Effects of anti-allergic compounds on histamine secretion by isolated intestinal mast cells, J. Immun.128, 2481–2486 (1982).PubMedGoogle Scholar
  13. [13]
    K.E. Barrett, K.B.P. Leung andF.L. Pearce,Histamine secretion and the effect of anti-allergic compounds on peritoneal mast cells of the rat, mouse and hamster, Br. J. Pharmac.78, 58P (1983).Google Scholar
  14. [14]
    W. Kazimierczak andB. Diamant,Mechanisms of histamine release in anaphylactic and anaphylactoid reactions, Prog. Allergy24, 295–365 (1978).PubMedGoogle Scholar
  15. [15]
    A. Sydbom andB. Uvnäs,Potentiation of anaphylactic histamine release from isolated rat pleural mast cells by rat serum phospholipids, Acta physiol. scand.97, 222–232 (1976).PubMedGoogle Scholar
  16. [16]
    R.P. Siraganian andK.A. Hazard,Mechanisms of mouse mast cell activation and inactivation for IgE-mediated histamine release J. Immun.122, 1719–1725 (1979).PubMedGoogle Scholar
  17. [17]
    A.D. Befus, F.L. Pearce, J. Gauldie, P. Horsewood andJ. Bienenstock,Mucosal mast cells I. Isolation and functional characteristics of rat intestinal mast cells, J. Immun.128, 2475–2480 (1982).PubMedGoogle Scholar
  18. [18]
    F.L. Pearce andM. Ennis,Isolation and some properties of mast cells from the mesentery of the rat and guinea pig, Agents and Actions10, 124–131 (1980).PubMedGoogle Scholar
  19. [19]
    M. Ennis,Histamine release from human pulmonary mast cells, Agents and Actions12, 60–63 (1982).PubMedGoogle Scholar
  20. [20]
    K.E. Barrett andF.L. Pearce,A comparative study of histamine secretion from rat peritoneal and pleural mast cells, Agents and Actions12, 186–188 (1982).PubMedGoogle Scholar
  21. [21]
    C.M.S. Fewtrell andB.D. Gomperts,Quercetin, a novel inhibitor of Ca 2+ influx and exocytosis in rat peritoneal mast cells, Biochim. biophys. Acta469, 52–60 (1977).PubMedGoogle Scholar
  22. [22]
    J. Tas andR.G. Berndsen,Does heparin occur in mucosal mast cells of the rat small intestine? J. Histochem. Cytochem.25 1058–1062 (1977).PubMedGoogle Scholar
  23. [23]
    E.J. Ruitenberg andA. Elgersma,Absence of intestinal mast cell response in congenitally athymic mice during Trichinella spiralis infection, Nature264, 258–260 (1976).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1984

Authors and Affiliations

  • K. E. Barrett
    • 1
  • D. H. Pluznik
    • 2
  • D. D. Metcalfe
    • 1
  1. 1.Allergic Diseases Section, Laboratory of Clinical InvestigationNational Institute of Allergy and Infectious DiseasesBethesdaUSA
  2. 2.Laboratory of Microbiology and Immunology, National Institute of Dental ResearchNational Institutes of HealthBethesdaUSA

Personalised recommendations