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, Volume 14, Issue 3–4, pp 468–474 | Cite as

Mechanism of histamine release from rat isolated peritoneal mast cells by dextran: The role of immunoglobulin E

  • T. H. P. Hanahoe
Histamine Release and Pathological States

Abstract

In vivo passive sensitization of rat peritoneal mast cells withNippostrongylus braziliensis antiserum or rat monoclonal myeloma IgE greatly enhanced histamine releasein vitro by dextran or anti IgE, but did not alter release by compound 48/80 or A23187. Conversely, removal of IgE from the cells by acid pH abolished histamine release by dextran and anti IgE but did not impair release by compound 48/80. Whereas, histamine release from cells isolated from rats genetically resistant to dextran (NR rats) by anti IgE was potentiated by passive sensitization, dextran was unable to stimulate secretion from control or sensitized NR cells. The results suggest that dextran releases histamine by interaction with cell-fixed IgE and that the NR mast cell membrane lacks the ability to interpret this stimulus.

Keywords

Cell Membrane Mast Cell Histamine Myeloma Dextran 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    J.H. Baxter andR. Adamik,Differences in requirements and actions of various histamine-releasing agents, Biochem. Pharmac.27, 497–503 (1978).CrossRefGoogle Scholar
  2. [2]
    G.W. Read, M. Knoohuizen andA. Goth,Relationship between phosphatidylserine and cromolyn in histamine release, Europ. J. Pharmacol.42, 171–177 (1977).CrossRefGoogle Scholar
  3. [3]
    G.W. Read andE.F. Kiefer,Benzalkonium chloride: selective inhibitor of histamine release induced by compound 48/80 and other polyamines, J. Pharmac. exp. Ther.211, 711–715 (1979).Google Scholar
  4. [4]
    G.W. Read, S.M. Hong andE.F. Kiefer,Competitive inhibition of 48/80 induced histamine release by benzalkonium chloride and its analogs and the polamine receptor in mast cells. J. Pharmac. exp. Ther.222, 652–657 (1982).Google Scholar
  5. [5]
    F.L. Pearce,Calcium and histamine secretion from mast cells, Prog. Med. Chem.19, 59–109 (1982).PubMedGoogle Scholar
  6. [6]
    A. Goth, H.R. Adams andM. Knoohuizen,Phosphatidylserine: selective enhancer of histamine release, Science173, 1034–1035 (1971).PubMedGoogle Scholar
  7. [7]
    T.H.P. Hanahoe, A. Holliman, D. Gordon andW. Wieczorek,Disodium cromoglycate and the dextran response in rats, J. Pharm. Pharmac.24, 666–667 (1972).Google Scholar
  8. [8]
    A. Goth andM. Knoohuizen,Genetically conditioned dextran co-factor in the rat, Fed. Proc.25, 692 (1966).Google Scholar
  9. [9]
    T.H.P. Hanahoe andW. Paska,Reaginic antibodies and the dextran reaction in the rat, Agents and Actions9, 92–93 (1979).PubMedGoogle Scholar
  10. [10]
    I. Moodley, J.L. Mongar andJ.C. Foreman,Histamine release induced by dextran: the nature of the dextran receptor, Eurp. J. Pharmac.83, 69–81. (1982).CrossRefGoogle Scholar
  11. [11]
    A. Goth,Inhibition of anaphylactoid oedema in the rat by 2-deoxyglucose, A. J. Physiol.197, 1056–1059 (1959).Google Scholar
  12. [12]
    A. Goth, W.L. Nash, M. Nagler andJ. Holman,Inhibition of histamine release in experimental diabetes, Am. J. Physiol.191, 25–28 (1957).PubMedGoogle Scholar
  13. [13]
    T.H.P. Hanahoe, T. Tanner andG.B. West,Resistance of rats to the potentiating action of phosphatidylserine on dextran responses, J. Pharm. Pharmac.25, 429–431 (1973).Google Scholar
  14. [14]
    W. Dias da Silva andA.D. Lemos Fernandes,Study of the mechanism of inhibition produced by hexoses on histamine release activity of dextran, Experientia21, 96–97 (1965).Google Scholar
  15. [5]
    A. Goth, Mechanism of histamine release by polymeric compounds. InMechanisms of Release of Biogenic Amines, p. 371. (EdsVon Euler, Rossell andUvnas) Pergamon Press, Oxford 1966.Google Scholar
  16. [16]
    C. Isersky, A. Kulczycki andH. Metzger,Isolation of IgE from reaginic rat serum, J. Immun.112, 1909–1919 (1974).PubMedGoogle Scholar
  17. [17]
    P.A. Shore, A. Burkhalter andV.H. Cohn,A method for the fluorometric assay of histamine in tissues, J. Pharmac. exp. Ther.127, 182–186 (1959).Google Scholar
  18. [18]
    A.H. Anton andD.F. Sayre,A modified fluorimetric procedure for tissue histamine and its distribution in various tissues, J. Pharmac. exp. Ther.166, 285–292 (1969).Google Scholar
  19. [19]
    T. Ishizaka andI. Ishizaka,Mechanisms of passive sensitisation. IV. Dissociation of IgE molecules from basophil receptors at acid pH, J. Immun.112, 1078–1084 (1977).Google Scholar
  20. [20]
    A. Kulczycki andH. Metzger,The interaction of IgE with basophilic leukaemia cells. II. Quantitative aspects of the binding reaction, J. exp. Med.140, 1676–1695 (1974).CrossRefPubMedGoogle Scholar
  21. [21]
    P. Stahl Skov,Removal and refixation of surface Ig from isolated rat mast cells, Scand. J. Immunol.6, 1015–1019 (1977).PubMedGoogle Scholar
  22. [22]
    J.C. Foreman, L.G. Garland andJ.L. Mongar, The role of calcuim in secretory processes: model studies in mast cell. In calciumin Biological Systems, 30th Symp. Soc. for Experimental Biology, p. 193. Cambridge University Press, Cambridge 1976.Google Scholar
  23. [23]
    Y. Morita andR.P. Siraganian,Inhibition of IgE mediated histamine release from rat basophilic leukaemia cells and rat mast cells by inhibitors of transmethylation, J. Immun.127, 1339–1344 (1981).PubMedGoogle Scholar
  24. [24]
    F. Hirata andJ. Axelrod,Phospholipid methylation and biological signal transmission, Science209, 1082–1090 (1980).PubMedGoogle Scholar
  25. [25]
    F. Hirata, J. Axelrod andF.T. Crews,Concanavalin A stimulates phospholipid methylation and phosphatidylserine decarboxylation in rat mast cells, Proc. Natn. Acad. Sci. USA76, 4813–4816 (1979).Google Scholar
  26. [26]
    R.I. Ludowyke, G.B. West, J.R. Harris andT.H.P. Hanahoe,A possible link between the ability of rat isolated peritoneal mast cells to release histamine when challenged with dextran and the proteins in their plasma membranes, Int. Archs Allergy appl. Immun.68, 188–191 (1982).Google Scholar
  27. [27]
    R.P. Siraganian andP.A. Siraganian,Mechanism of action of concanavalin A on human basophils, J. Immun.114, 886–893 (1975).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1984

Authors and Affiliations

  • T. H. P. Hanahoe
    • 1
  1. 1.Department of Paramedical SciencesNorth East London PolytechnicLondon

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