Rats were exposed by inhalation to the technically highest administrable concentration of 230 mg Pigment Yellow 17/m3 air for 4 h. Inhalability of the dust was guaranteed by a mass-median aerodynamic diameter of 1.0–1.1 μm. For 14 days after exposure, urine and serum samples were analysed for 3,3′-dichlorobenzidine, the parent carcinogenic amine of the test compound. No 3,3′-dichlorobenzidine could be detected either in urine or blood, the detection limit being 5 ng/ml for both media. Based on the results of this study there is no evidence for metabolic cleavage of Pigment Yellow 17 to 3,3′-dichlorobenzidine in the rat.
Pigment Yellow 17 3,3′-Dichlorobenzidine Biotransformation Gas chromatography
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