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Imipenem resistance inEnterobacter

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Abstract

Blood cultures obtained on two separate occasions from a 37-year-old male who received multiple antibiotics (including imipenem) for treatment of repeated episodes of intraabdominal abscesses and bacteremia yielded two isolates ofEnterobacter with reduced susceptibility to imipenem, extended-spectrum cephalosporins, penicillins and aztreonam. Both isolates were unstable, giving rise to different colony types, each of which produced a single, non-inducible Bush group 1 β-lactamase (pI=9.6) that hydrolyzed imipenem. Outer membrane proteins were analyzed but no differences were detected between strains with different levels of imipenem resistance. Three-dimensional tests performed in conjunction with disk diffusion susceptibility tests provided a rapid and convenient means of detecting the production of imipenem-hydrolyzing enzymes by theEnterobacter strains. These isolates provided additional evidence that overproduction of the group 1 cephalosporinase ofEnterobacter can contribute to resistance to imipenem.

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Authors and Affiliations

  1. Department of Medical Microbiology, Creighton University School of Medicine, 2500 California Plaza, 68178, Omaha, Nebraska, USA

    K. S. Thomson & C. C. Sanders

  2. Department of Medicine, St. Francis Medical Center, Trenton, New Jersey, USA

    H. Chmel

Authors
  1. K. S. Thomson
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  2. C. C. Sanders
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  3. H. Chmel
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Thomson, K.S., Sanders, C.C. & Chmel, H. Imipenem resistance inEnterobacter . Eur. J. Clin. Microbiol. Infect. Dis. 12, 610–613 (1993). https://doi.org/10.1007/BF01973639

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