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Western blot analysis of antibody responses of young infants to pertussis infection

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Abstract

Western blot and agglutination techniques were used to analyze the antibody responses toBordetella pertussis in 27 infants less than six month of age with presumed pertussis infection. The antibody response to theBordetella pertussis adhesins filamentous hemagglutinin, pertactin and agglutinogens, and to theBordetella pertussis toxins pertussis toxin and adenylate cyclase-hemolysin were compared. Infection induced intense antibody responses to filamentous hemagglutinin, pertussis toxin and adenylate cyclase-hemolysin. Antibodies to agglutinogens were never detected, and antibodies to pertactin were rarely detected in infected infants' sera. Therefore, determination of anti-agglutinogens levels only is not suitable for the serological diagnosis of pertussis in young infants. Use of purified filamentous hemagglutinin, pertussis toxin and adenylate cyclase-hemolysin in Western blot analysis may improve the serodiagnosis ofBordetella infections. However, care must be exercised in distinguishing between the antibody response in young infants and maternally derived antibodies.

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References

  1. Geller RJ The pertussis syndrome: a persistent problem. Pediatric Infectious Disease Journal 1984, 3: 182–185.

    Google Scholar 

  2. Bass JW, Stephenson SR The return of pertussis. Pediatric Infectious Disease Journal 1987, 6: 141–144.

    PubMed  Google Scholar 

  3. Sato Y, Sato H: Animal model to pertussis. In: Wardlaw AC, Parton R (ed): Pathogenesis and immunity in pertussis. J. Wiley, 1988, p. 309–325.

  4. Leninger E, Roberts M, Kenimer JG, Charles IG, Fairweather N, Novotny P, Brennan MJ Pertactin, an Arg-Gly-Asp-containingBordetella pertussis surface protein that promotes adherence to mammalian cells. Proceedings of National Academy of Sciences of the USA 1991, 88: 345–350.

    Google Scholar 

  5. Shahin RD, Brennan MJ, Li ZM, Meade BD, Manclark CR Characterization of the protection capacity and immunogenicity of the 69-kD outer membrane protein ofBordetella pertussis. Journal of Experimental Medicine 1990, 171: 63–69.

    Article  PubMed  Google Scholar 

  6. Hanski E, Coote JG: Bordetella pertussis adenylate cyclase toxin. In: Alouf JE, Freers JH (ed.): Bacterial protein toxins. Academic Press 1991, p. 349–366.

  7. Guiso N, Szatanik M, Rocancourt M Protective activity ofBordetella adenylate cyclase-hemolysin against bacterial colonization. Microbial Pathogenesis 1991, 11: 423–431.

    Article  PubMed  Google Scholar 

  8. Thomas MG, Ashworth E, Miller E, Lambert HP Serum IgG, IgA and IgM responses to pertussis toxin, filamentous hemagglutinin and agglutinogens 2 and 3 after infection withBordetella pertussis and immunization with whole-cell pertussis vaccine. Journal of Infectious Disease 1989, 160: 838–845.

    PubMed  Google Scholar 

  9. Redd SC, Rumschlag HS, Biellik RJ, Sanden GN, Reimer CB, Cohen ML Immunoblot analysis of humoral immune responses following infection withBordetella pertussis or immunization with diphteriatetanus-pertussis vaccine. Journal of Clinical Microbiology 1989, 26: 1373–1377.

    Google Scholar 

  10. Thomas MG, Redhead K, Lambert HP Human serum antibody responses toBordetella pertussis infection and pertussis vaccination. Journal of Infectious Disease 1989, 159: 211–218.

    Google Scholar 

  11. Arciniega JL, Hewlett EL, Johnson FD, Deforest A, Wassilak SGF, Onorato IM, Manclark CR, Burns DL Human serologic response to envelope-associated proteins and adenylate cyclase toxin ofBordetella pertussis. Journal of Infectious Diseases 1991, 163: 135–142.

    PubMed  Google Scholar 

  12. Farfel Z, Konen S, Wiertz E, Klapmuts R, Addy PAK, Hanski E Antibodies toBordetella pertussis adenylate cyclase are produced in man during pertussis infection and after vaccination. Medical Microbiology 1990, 32: 173–177.

    PubMed  Google Scholar 

  13. Olin P New conclusions and lessons learned from the vaccine trial in Sweden. In: Manclark CR (ed): Proceedings of the Sixth International Symposium on Pertussis. Department of Health and Human Services, Bethesda, MD, 1990, p. 299–301.

    Google Scholar 

  14. Relyveld E, Oato NH, Guérin N, Coursaget P, Huet M, Gupta RK Determination of circulating antibodies directed to pertussis toxin and to agglutinogens in children vaccinated with either the whole-cell or component vaccine in France, Japan and Senegal. Vaccine 1991, 9: 843–850.

    Article  PubMed  Google Scholar 

  15. Arico B, Rappuoli R Bordetella parapertussis andBordetella bronchiseptica contain transcriptionally silent pertussis toxin genes. Journal of Bacteriology 1987, 169: 2847–2853.

    PubMed  Google Scholar 

  16. Meade BD, Mink CM, Manclark CR Serodiagnosis of pertussis. In: Manclark CR (ed): Proceedings of the Sixth International Symposium on Pertussis. Department of Health and Human Services, Bethesda, MD, 1990, p. 322–329.

    Google Scholar 

  17. Pittman M Pertussis toxin: the cause of the harmful effects and prolonged immunity of whooping cough. A hypothesis. Reviews of Infectious Diseases 1979, 1: 401–413.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institut Pasteur, Unité de Bactériologie Moléculaire et Médicale, 28 Rue du Dr. Roux, 75724, Paris Cedex 15, France

    N. Guiso & E. Grimprel

  2. Consultation de Pédiatrie, Pathologie Infectieuse et Tropicale, Urgences Pédiatriques, Hôpital d'Enfants Armand Trousseau, 26 Avenue du Dr. Arnold Netter, 75571, Paris Cedex 12, France

    E. Grimprel, I. Anjak & P. Bégué

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  1. N. Guiso
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  2. E. Grimprel
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  3. I. Anjak
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  4. P. Bégué
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Guiso, N., Grimprel, E., Anjak, I. et al. Western blot analysis of antibody responses of young infants to pertussis infection. Eur. J. Clin. Microbiol. Infect. Dis. 12, 596–600 (1993). https://doi.org/10.1007/BF01973637

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