Archives of Toxicology

, Volume 64, Issue 4, pp 343–344 | Cite as

Detection of diepoxybutane-induced DNA-DNA crosslinks by cesium trifluoracetate (CsTFA) density-gradient centrifugation

  • C. Ristau
  • S. Deutschmann
  • R. J. Laib
  • H. Ottenwälder
Letter to the Editor

Keywords

Centrifugation Cancer Research Cesium Trifluoracetate 

Abbreviations

DDC

DNA-DNA crosslinks

CsTFA

Cesiumtrifluoracetate

BD

butadiene

DEB

diepoxybutane

References

  1. Carter C, Britton VJ, Haff L (1983) CsTFA™: a centrifugation medium for nucleic acid isolation and purification. In: Biotechniques, pp 142–146Google Scholar
  2. Jelitto B, Vangala RR, Laib RJ (1989) Species differences in DNA damage by butadiene: Role of diepoxybutane. Arch Toxicol Suppl 13: 246–249PubMedGoogle Scholar
  3. Kohn KW, Spears CL, Doty P (1966) Inter-strand crosslinking of DNA by nitrogen mustard. J Mol Biol 19: 266–288PubMedGoogle Scholar
  4. Kreiling R, Laib RJ, Bolt HM (1986) Alkylation of nuclear proteins and DNA after exposure of rats and mice to 1,4−14-C 1,3-butadiene. Toxicol Lett 30: 131–135PubMedGoogle Scholar
  5. Laib RJ, Deutschmann S, Jelitto B, Vangala RR, Kreiling R (1989) Species differences in pharmacokinetics, metabolism, and DNA binding of inhaled 1,3-butadiene. In: Mohr et al. (eds) Assessment of inhalation hazards: integration and extrapolation using diverse data. Springer-Verlag, New York, pp 189–199Google Scholar
  6. Lawley PD, Brookes P (1967) Interstrand cross-linking of DNA by bifunctional alkylating agents. J Mol Biol 25: 143–160PubMedGoogle Scholar
  7. Malvoisin E, Robertfroid M (1982) Hepatic microsomal (metabolism of 1,3-butadiene. Xenobiotica 12: 137–144PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • C. Ristau
    • 1
  • S. Deutschmann
    • 1
  • R. J. Laib
    • 1
  • H. Ottenwälder
    • 1
  1. 1.Institut für Arbeitsphysiologie and der Universität DortmundDortmund 1Federal Republic of Germany

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