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, Volume 39, Supplement 1, pp C163–C165 | Cite as

Release of cell surface proteoglycan from chondrocytes by interleukin-1

  • R. L. Goldberg
  • S. Spirito
  • J. R. Doughty
  • G. Di Pasquale
Osteoarthritis

Abstract

A cell culture model was developed to assess factors that can protect cartilage from recombinant human interleukin-1α (IL-1)-induced breakdown. IL-1 (0.01–10 ng/ml) caused a dose-dependent release of cell surface proteoglycan into the medium of bovine and rabbit articular chondrocytes. Sulfated glycosaminoglycan (S-GAG) was detected in the medium and on the cell surface by a dimethlymethylene blue assay. The redistribution of the cell surface S-GAG to the medium compartment required protein synthesis because it was inhibited by cycloheximide and was time-dependent (>5 h). Although the release was most likely due tode novo synthesis of proteases, standard protease inhibitors failed to prevent the release even when used in combination. TGF-β and IGF-I increased the amount of S-GAG in both the medium and cell surface compartments, but did not protect from IL-1-induced release. This method has an advantage over a cartilage model of IL-1-induced matrix release because the drug and cytokine exposure time is reduced and the variability is less.

Keywords

Cell Surface Protease Inhibitor Cycloheximide Glycosaminoglycan Culture Model 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • R. L. Goldberg
    • 1
  • S. Spirito
    • 1
  • J. R. Doughty
    • 1
  • G. Di Pasquale
    • 1
  1. 1.Research Department, Pharmaceuticals DivisionCIBA-GEIGY CorporationSummitUSA

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