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, Volume 39, Supplement 1, pp C70–C72 | Cite as

The effects of anti-inflammatory and antiallergic drugs on the release of IL-1β and TNF-α in the human whole blood assay

  • D. A. Hartman
  • S. J. Ochalski
  • R. P. Carlson
Regulation of Cytokinses

Abstract

Heparinized human whole blood was evaluated as a model to study the effects of various classes of anti-inflammatory drugs on IL-1β and TNF-α release from leukocytes. Human whole blood was stimulated with zymosan (1.5 mg/ml) or LPS (5 μg/ml) to induce significant cytokine release. As previously reported, the 5-lipoxygenase/cyclooxygenase (5-LO/CO) inhibitor, SKF86002 (30 μM), significantly inhibited both IL-1β and TNF-α release using either stimulus. In contrast, the cyclooxygenase (CO) inhibitors (naproxen and ibuprofen) and the lipoxygenase (5-LO) inhibitors (zileuton, L-663536 and BWA4C) did not effect IL-1β or TNF-α release/biosynthesis. Isoproterenol (β-agonist), rolipram (a PDE-IV inhibitor), and IBMX (a nonselective PDE inhibitor), significantly inhibited TNF-α but not IL-1β in the LPS model while having no effect in the zymosan model. This human whole blood assay is a unique and rapid method which can be used to identify novel inhibitors of IL-1β and TNF-α release/biosynthesis.

Keywords

Ibuprofen Naproxen Isoproterenol Rapid Method Cytokine Release 

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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • D. A. Hartman
    • 1
  • S. J. Ochalski
    • 1
  • R. P. Carlson
    • 1
  1. 1.Wyeth-Ayerst Research, CN-8000PrincetonUSA

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