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Agents and Actions

, Volume 39, Supplement 1, pp C8–C10 | Cite as

Inflammatory mediator changes in cotton-top tamarins (CTT) after SC-41930 anti-colitic therapy

  • N. Clapp
  • M. Henke
  • R. Hansard
  • R. Carson
  • R. Walsh
  • D. Widomski
  • C. Anglin
  • D. Fretland
Lipid Mediators

Abstract

Use of the CTT model provides insight into the inflammatory mediator contribution in the pathogenesis of idiopathic colitis. To evaluate anti-colitic efficacy, the leukotriene B4 receptor antagonist and anti-inflammatory agent, SC-41930, was administered (10 mg/kg BW by gavage BID) for 8 weeks to CTTs with histologically confirmed persistent and defined active colitis. The inflammatory mediators LTB4, PGE2, TXB2, and PAF were assayed in colonic dialysate that was collected after 11/2 h from four CTTs pre-, mid-, and post-treatment, frozen at −70°C, and analyzed by RIA after HPLC purification. LTB4 levels were lower at mid- and post-treatment and had little inter-animal variation post-treatment. PGE2 and PAF levels were elevated during SC-41930 treatment, but there was a trend towards lower thromboxane B2 levels. Reduced LTB4 (PMN degranulation and chemotaxis) and increased PGE2 (mucosal-protective effect) may, in part, explain the observed efficacy of SC-41930 in active tamarin colitis.

Keywords

PGE2 Inflammatory Mediator LTB4 HPLC Purification Idiopathic Colitis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • N. Clapp
    • 1
  • M. Henke
    • 1
  • R. Hansard
    • 1
  • R. Carson
    • 1
  • R. Walsh
    • 2
  • D. Widomski
    • 2
  • C. Anglin
    • 2
  • D. Fretland
    • 2
  1. 1.Marmoset Research Center at Oak Ridge (MARCOR)Oak Ridge Associated UniversitiesOak RidgeUSA
  2. 2.Immunoinflammatory Diseases Research, Searle Research and DevelopmentSkokieUSA

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