Agents and Actions

, Volume 39, Supplement 1, pp C8–C10 | Cite as

Inflammatory mediator changes in cotton-top tamarins (CTT) after SC-41930 anti-colitic therapy

  • N. Clapp
  • M. Henke
  • R. Hansard
  • R. Carson
  • R. Walsh
  • D. Widomski
  • C. Anglin
  • D. Fretland
Lipid Mediators

Abstract

Use of the CTT model provides insight into the inflammatory mediator contribution in the pathogenesis of idiopathic colitis. To evaluate anti-colitic efficacy, the leukotriene B4 receptor antagonist and anti-inflammatory agent, SC-41930, was administered (10 mg/kg BW by gavage BID) for 8 weeks to CTTs with histologically confirmed persistent and defined active colitis. The inflammatory mediators LTB4, PGE2, TXB2, and PAF were assayed in colonic dialysate that was collected after 11/2 h from four CTTs pre-, mid-, and post-treatment, frozen at −70°C, and analyzed by RIA after HPLC purification. LTB4 levels were lower at mid- and post-treatment and had little inter-animal variation post-treatment. PGE2 and PAF levels were elevated during SC-41930 treatment, but there was a trend towards lower thromboxane B2 levels. Reduced LTB4 (PMN degranulation and chemotaxis) and increased PGE2 (mucosal-protective effect) may, in part, explain the observed efficacy of SC-41930 in active tamarin colitis.

Keywords

PGE2 Inflammatory Mediator LTB4 HPLC Purification Idiopathic Colitis 

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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • N. Clapp
    • 1
  • M. Henke
    • 1
  • R. Hansard
    • 1
  • R. Carson
    • 1
  • R. Walsh
    • 2
  • D. Widomski
    • 2
  • C. Anglin
    • 2
  • D. Fretland
    • 2
  1. 1.Marmoset Research Center at Oak Ridge (MARCOR)Oak Ridge Associated UniversitiesOak RidgeUSA
  2. 2.Immunoinflammatory Diseases Research, Searle Research and DevelopmentSkokieUSA

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