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, Volume 5, Issue 5, pp 431–439 | Cite as

Diamine oxydase in rabbit small intestine: Separation from a soluble monoamine oxidase, properties and pathophysiological significance in intestinal ischemia

  • Jürgen Kusche
  • Hans Richter
  • Johanna Schmidt
  • Rudolf Hesterberg
  • Anne Friedrich
  • Wilfried Lorenz
Histamine and Kinins

Abstract

From all mammals investigated so far only in rabbits diamine oxidase could not be detected in any tissue except the gut. Thus this species was chosen for studying the physiological and pathophysiological function of this enzyme in the gastrointestinal tract.

By gel filtration on Sephadex G 50 and G 200 the enzyme was purified 100-fold, separated from a soluble monoamine oxidase, and the properties of the two enzymes were determined. Diamine oxidase from rabbit small intestine deaminated putrescine (K m =1.3×10−4M, pH-optimum 6.4–6.9) and histamine (K m =8×10−5M, pH-optimum 7.5), but not serotonin, and was inhibited by aminoguanidine, but not by pargyline. Soluble monoamine oxidase from rabbit small intestine catabolized serotonin (K m =1.8×10−4M, pH-optimum 8.8), but not putrescine and histamine, and was inhibited by pargyline, but not by aminoguanidine.

Based on its properties in vitro intestinal diamine oxidase could inactivate the vasoactive biogenic amine histamine in vivo. To confirm this hypothesis, in rabbits the small intestine was damaged severely by inducing total intestinal ischemia, which occurs as mesenteric infarction also in human subjects and is accompanied by histamine release. Treatment with aminoguanidine and ischemia killed the animals 3-times faster than ischemia alone, which supported our hypothesis on a protective role of intestinal diamine oxidase against histamine.

Keywords

Ischemia Serotonin Histamine Diamine Putrescine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1975

Authors and Affiliations

  • Jürgen Kusche
    • 1
  • Hans Richter
    • 1
  • Johanna Schmidt
    • 1
  • Rudolf Hesterberg
    • 1
  • Anne Friedrich
    • 1
  • Wilfried Lorenz
    • 1
  1. 1.Abteilung für Experimentelle Chirurgie und Pathologische Biochemie an der Chirurgischen Klinik der UniversitätMarburg a.d. LahnFederal Republic of Germany

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