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Naturally occurring low molecular weight inhibitors of neutral proteinases from PMN-granulocytes and of kallikreins

  • Histamine and Kinins
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Abstract

Human mucous secretions contain low molecular weight (M r ∼11,000) acid-stable inhibitors directed against elastase and cathepsin G from PMN-granulocytes. Important biochemical properties of these inhibitors are presented and their possible biological function is discussed. An inhibitor of glandular and plasma kallikreins preventing kinin-liberation from kininogen but not ester hydrolysis was obtained from rat kidney tubules. A molecular weight of about 4700 was estimated for this kallikrein-specific inhibitor (trypsin-induced kinin-liberation is not prevented).

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References

  1. Only key references are given; further references are available from the authors on request.

  2. M.J. Cline,The White Cell (Harvard University Press, Cambridge, Mass., 1975).

    Google Scholar 

  3. C.G. Van Arman andN.R. Bohidar,Role of the Kallikrein-Kinin System in Inflammation, in:Chemistry and Biology of the Kallikrein-Kinin System in Health and Disease (Eds. J.J. Pisano and K.F. Austen; U.S. Government Printing Office, Washington, D.C., DHEW Publ. No. (NIH) 76-791, 1976), pp. 471–481.

    Google Scholar 

  4. C.-B. Laurell,Relation Between Structure and Biologic Function of the Protease Inhibitors in the Extracellular Fluids, in:Protides of the Biological Fluids — 22nd Colloquium, vol. 22 (Ed. H. Peeters; Pergamon Press, Oxford, 1975), pp. 3–12.

    Google Scholar 

  5. K. Ohlsson andI. Olsson,The Neutral Proteases of Human Granulocytes. Isolation and Partial Characterization of Granulocyte Elastase, Eur. J. Biochem.42, 519–527 (1974).

    Google Scholar 

  6. P. Venge, I. Olsson andH. Odeberg,Cationic Proteins of Human Granulocytes. V. Interaction with Plasma Protease Inhibitors, Scand. J. clin. Lab. Invest.35, 737–744 (1975).

    Google Scholar 

  7. W. Schmidt, R. Egbring andK. Havemann,Effect of Elastase-like and Chymotrypsin-like Neutral Proteases from Human Granulocytes on Isolated Clotting Factors, Thromb. Res.6, 315–326 (1975).

    Google Scholar 

  8. A. Solomon, W. Schmidt andK. Haemann,Bence Jones Proteins and Light Chains of Immunoglobulins. XIII. Effect of Elastase-like and Chymotrypsin-like Neutral Proteases Derived from Human Granulocytes on Bence Jones Proteins, J. Immunology117, 1010–1014 (1976).

    Google Scholar 

  9. L.M. Greenbaum,Cellular Kininogenases and Leukokinins, in:Chemistry and Biology of the Kallikrein-Kinin System in Health and Disease (Eds. J.J. Pisano and K.F. Austen; U.S. Government Printing Office, Washington, D.C., DHEW Publ. (NIH) 76-791, 1976), pp. 455–462.

    Google Scholar 

  10. H.Z. Movat, F.M. Habal andD.R.L. Macmorine,The Cleavage of a Methionyl-Lysyl-Bradykinin-like Peptide from Kininogen by a Protease of Human Neutrophil Leucocyte Lysosomes, in:Kinins-Pharmacodynamics and Biological Roles, Adv. exp. Med. Biol., vol. 70 (Eds. F. Sicuteri, N. Back and G.L. Haberland; Plenum Press, New York, 1975), pp. 345–359.

    Google Scholar 

  11. K. Ohlsson, Granulocyte Collagenase and Elastase and Their Interactions with α1-Antitrypsin and α2-Macroglobulin, in:Proteases and Biological Control (Eds. E. Reich, D.B. Rifkin and E. Shaw; Cold Spring Harbor Laboraotry, 1975), pp. 591–602.

  12. K. Ohlsson andU. Åkesson, α1-Antichymotrypsin Interaction with Cationic Proteins from Granulocytes, Clin. Chim. Acta73, 285–291 (1976).

    Google Scholar 

  13. K. Ohlsson,Interaction Between Endogenous Proteases and Plasma Protease Inhibitors in vitro and in vivo, in:Bayer Symposium V — Proteinase Inhibitors (Eds. H. Fritz, H. Tschesche, L.J. Greene and E. Truscheit; Springer-Verlag, Berlin, 1974), pp. 96–105.

    Google Scholar 

  14. H. Schiessler, M. Arnhold andH. Friz,Characterization of Two Proteinase Inhibitors from Human Seminal Plasma and Spermatozoa, in:Bayer Symposium V — Proteinase Inhibitors (Eds. H. Fritz, H. Tschesche, L.J. Greene and E. Truscheit; Springer Verlag, Berlin, 1974), pp. 147–155.

    Google Scholar 

  15. O. Wallner, H. Fritz andK. Hochstrasser,An Acid-Stable Proteinase Inhibitor in Human Cervical Mucus, in:Protides of the Biological Fluids — 23rd Colloquium, vol. 23 (Ed. H. Peeters; Pergamon Press, Oxford, 1976), pp. 177–182.

    Google Scholar 

  16. K. Hochstrasser, R. Reichert, E Werle andH. Haendle,New Proteinase Inhibitors in the Secretions of Some Mucous Membranes of the Human Body, in:Protides of the Biological Fluids — 20th Colloquium, vol. 20 (Ed. H. Peeters; Pergamon Press, Oxford, 1973), pp. 417–424.

    Google Scholar 

  17. K. Ohlsson, H. Tegner andU. Åkesson,Isolation and Partial Characterization of a Low Molecular Weight Acid-Stable Protease Inhibitor from Human Bronchial Secretion, Hoppe Seyler's Z. physiol. Chem.358, 583–589 (1977).

    Google Scholar 

  18. H. Schiessler,Säurestabile Proteinase-Inhibitoren aus menschlichem Sperma und ihre Zielenzyme —Isolierung, Eigenschaften und Biologische Funktion, in:Proteinasen und Proteinase-Inhibitoren beim Menschen, Adv. Andrology, vol. 5 (Ed. C. Schirren Grosse Verlag, Berlin, 1976), pp. 45–102.

    Google Scholar 

  19. H. Schiessler, M. Arnhold, K. Ohlsson andH. Fritz,Inhibitors of Acrosin and Granulocyte Proteinases from Human Genital Tract Secretions, Hoppe Seyler's Z. physiol. Chem.357, 1251–1260 (1976).

    Google Scholar 

  20. H. Tegner andK. Ohlsson,Localization of a Low Molecular Weight Protease Inhibitor to Tracheal and Maxillary Sinus Mucosa, Hoppe Seyler's Z. physiol. Chem.358, 425–429 (1977).

    Google Scholar 

  21. K. Hochstrasser, G. Bretzel, E. Wachter andS. Heindl,The Amino Acid Sequence of the Double-Headed Proteinase Inhibitor from Canine Submandibular Glands, III. Sequencing Studies, Hoppe Seyler's Z. physiol. Chem.356, 1865–1877 (1975).

    Google Scholar 

  22. H. Tschesche, S. Kupfer, R. Klauser, E. Fink andH. Fritz,Structure, Biochemistry and Comparative Aspects of Mammalian Seminal Plasma Acrosin Inhibitors, in:Protides of the Biological Fluids — 23rd Colloquium, vol. 23 (Ed. H. Peeters; Pergamon Press, Oxford, 1976), pp. 255–266.

    Google Scholar 

  23. I. Kato, J. Schrode, K.A. Wilson andM. Laskowski, Jr,Evolution of Proteinase Inhibitors, in:Protides of the Biological Fluids — 23rd Colloquium, vol. 23 (Ed. H. Peeters; Pergamon Press, Oxford, 1976), pp. 235–243.

    Google Scholar 

  24. H. Fritz, E. Jaumann, R. Meister, P. Pasqay, K. Hochstrasser andE. Fink,Proteinase Inhibitors from Dog Submandibular Glands — Isolation, Amino Acid Composition, Inhibition Spectrum, in:Proc. Int. Res. Conf. on Proteinase Inhibitors, Munich, Nov. 4–6, 1970 (Eds. H. Fritz and H. Tschesche; W. de Gruyter, Berlin, 1971), pp. 257–270.

    Google Scholar 

  25. H. Schiessler, K. Ohlsson, I. Olsson, M. Arnhold, Y. Birk andH. Fritz,Elastases from Human and Canine Granulocytes, II. Interaction with Protease Inhibitors of Animal, Plant and Microbial Origin, Hoppe Seyler's Z. physiol. Chem.358, 53–58 (1977).

    Google Scholar 

  26. T. Ikenaka, S. Odani andT. Koide,Chemical Structure and Inhibitory Activities of Soybean Proteinase Inhibitors, in:Bayer Symposium V — Proteinase Inhibitors (Eds. H. Fritz, H. Tschesche, L.J. Greene and E. Truscheit; Springer Verlag, Berlin, 1974), pp. 325–343.

    Google Scholar 

  27. A. Okura, H. Morishima, T. Takita, T. Aoyagi, T. Takeuchi andH. Umezawa,The Structure of Elastatinal, an Elastase Inhibitor of Microbial Origin, J. Antibiotics28, 337–339 (1975).

    Google Scholar 

  28. H. Fritz,Glandular and Plant Kallikrein Inhibitors, in:Chemistry and Biology of the Kallikrein-Kinin System in Health and Disease (Eds. J.J. Pisano and K.F. Austen; US. Government Printing Office, Washington, D.C., DHEW Publ. No. (NIH) 76-791, 1977), pp. 181–193.

    Google Scholar 

  29. P.C. Harpel,Circulating Inhibitors of Human Plasma Kallikrein, in:Chemistry and Biology of the Kallikrein-Kinin System in Health and Disease (Eds. J.J. Pisano and K.F. Austen; U.S. Government Printing Office, Washington, D.C., DHEW Publ. No. (NIH) 76-791, 1977), pp. 169–178.

    Google Scholar 

  30. C.E. Burrowes, F.M. Habal andH.Z. Movat,The Inhibition of Human Plasma Kallikrein by Antithrombin III, Thromb. Res.7, 175–183 (1975).

    Google Scholar 

  31. H. Fritz, Human α1-Antitrypsin: Progressive Inhibitor of Plasma and Pancreatic Kallikrein, in:Chemistry and Biology of the Kallikrein-Kinin System in Health and Disease (Eds. J.J. Pisano and K.F. Austen; U.S. Government Printing Office, Washington, D.C., DEHW Publ. No. (NIH) 76-791, 1977), p. 179.

    Google Scholar 

  32. R. Huber, D. Kukla, W. Steigemann, J. Deisenhofer andA. Jones,Structure of the Complex Formed by Bovine Trypsin and Bovine Pancreatic Trypsin Inhibitor, Refinement of the Crystal Structure Analysis, in:Bayer Symposium V — Proteinase Inhibitors (Eds. H. Fritz, H. Tschesche, L.J. Greene and E. Truscheit; Springer Verlag, Berlin, 1974), pp. 497–512.

    Google Scholar 

  33. G. Siqueira andW.T. Beraldo,Attempts to Isolate a Kallikrein Inhibitor from Rat Salivary Gland, Agents and Actions3, 323–325 (1973).

    Google Scholar 

  34. R. Geiger andK. Mann,A Kallikrein-Specific Inhibitor in Rat Kidney Tubules, Hoppe Seyler's Z. physiol. Chem.357, 553–558 (1976).

    Google Scholar 

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Authors and Affiliations

  1. Abteilung für Klinische Chemie und Klinische Biochemie in der Chirurgischen Klinik der Universität München, D-8000, München 2, Fed. Rep. of Germany

    Hans Fritz, Hans Schiessler & Reinhard Geiger

  2. Department of Clinical Chemistry and Surgery, Malmö General Hospital, S-21401, Malmö, Sweden

    Kjell Ohlsson

  3. HNO-Klinik der Universität München, D-8000, München 2, Fed. Rep. of Germany

    Karl Hochstrasser

Authors
  1. Hans Fritz
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  2. Hans Schiessler
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  3. Reinhard Geiger
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  4. Kjell Ohlsson
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  5. Karl Hochstrasser
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Fritz, H., Schiessler, H., Geiger, R. et al. Naturally occurring low molecular weight inhibitors of neutral proteinases from PMN-granulocytes and of kallikreins. Agents and Actions 8, 57–64 (1978). https://doi.org/10.1007/BF01972403

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