Experientia

, Volume 38, Issue 7, pp 786–787 | Cite as

Enzyme immunoassay for arginine vasopressin

  • T. Uno
  • K. Uehara
  • K. Motomatsu
  • E. Ishikawa
  • K. Kato
Specialia Chimica, Biochimica

Summary

A highly sensitive enzyme immunoassay for arginine vasopressin (AVP) was described. The enzyme used was β-D-galactosidase fromEscherichia coli, and it was coupled to AVP by using the N-hydroxysuccinimide ester of N-(4-carboxycyclohexylmethyl)-maleimide. The complex was then utilized for the enzyme immunoassay, which could detect 0.5 fmoles (or 0.5 pg) of AVP in the assay tube.

Keywords

Enzyme Ester Arginine Vasopressin Enzyme Immunoassay 

References

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    E. Ishikawa, Y. Yamada, Y. Hamaguchi, S. Yoshitake, K. Shiomi, T. Ota, Y. Yamamoto and K. Tanaka, in: Enzyme labeled immunoassay of hormones and drugs, p. 45. Ed. S. B. Pal. Walter de Gruyter, Berlin 1978.Google Scholar

Copyright information

© Birkhäuser Verlag 1982

Authors and Affiliations

  • T. Uno
    • 1
    • 2
  • K. Uehara
    • 1
    • 2
  • K. Motomatsu
    • 1
    • 2
  • E. Ishikawa
    • 1
    • 2
  • K. Kato
    • 1
    • 2
  1. 1.Department of Anesthesiology and BiochemistryMedical College of MiyazakiMiyazaki(Japan)
  2. 2.Department of BiochemistryInstitute for Developmental ResearchKasugai, Aichi(Japan)

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