Enzyme immunoassay for arginine vasopressin
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A highly sensitive enzyme immunoassay for arginine vasopressin (AVP) was described. The enzyme used was β-D-galactosidase fromEscherichia coli, and it was coupled to AVP by using the N-hydroxysuccinimide ester of N-(4-carboxycyclohexylmethyl)-maleimide. The complex was then utilized for the enzyme immunoassay, which could detect 0.5 fmoles (or 0.5 pg) of AVP in the assay tube.
KeywordsEnzyme Ester Arginine Vasopressin Enzyme Immunoassay
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