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, Volume 6, Issue 1–3, pp 219–227 | Cite as

Adjuvant-induced arthritis in four inbred strains of rats. An in vitro study of peripheral T and B lymphocytes

  • A. Kahan
  • F. Perlik
  • A. Le Go
  • F. Delbarre
  • J. P. Giroud
Symposium 4 General Aspects of Inflammation I

Abstract

The lymphoblastic response (LTT) to non-specific mitogens (PHA, PWM and CoA) of peripheral lymphocytes was investigated at days 0, 7, 14, 21 and 28 after adjuvant injection in four strains of inbred rats: Wistar (WAG), Long Evans (LE), Lewis (LEW) and Brown Norway (BN). LTT was assessed by using 18 hours H3 TdR incorporation in 5 days cultures of whole blood (micromethod).

The statistical treatment of data, using principal components multifactorial analysis and analysis of variance showed a striking difference between strains.

In control animals the responses to PHA and PWM were correlated and were higher in LE and WAG than in LEW and BN (BN=LEW<LE=WAG). The response to ConA was independent of that to the other mitogens. It was generally low, but significantly higher in LEW and BN than in WAG and LE.

In adjuvant-injected animals the responses to PHA and PWM were still correlated, but modified compared to control: in LE and LEW, but not in WAG and BN, a marked decrease of the response was found, reaching a minimum value within days 7 and 14. In the same time the response to ConA increased in the four strains, later in LE than in the others. However the intensity of the ConA response varied from one strain to another: it was constantly low in LE and WAG compared to LEW and BN. So the most striking modification of LTT were observed in LE and LEW, which both developed the most severe arthritis. However these different behaviours after adjuvant injection were not explained by the initial level of LTT to the different mitogens.

These data suggest that the development of intense arthritis is associated with the proliferation and the release into the blood stream of a lymphocyte subpopulation, which exhibits a low response to PHA and PWM and a high response to ConA. These LTT modifications are not paralled by quantitative variations of B-cells assessed by surface Ig immunofluorescent staining and EAC rosetting.

Keywords

Arthritis Immunofluorescent Staining Blood Stream Inbred Strain Peripheral Lymphocyte 

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References

  1. [1]
    Berney, S., Bishko, F., and Quagliato, F.,Distribution of normal and sensitized lymphoid cells with adjuvant-induced arthritis, Arthritis Rheum.14, 370 (abstract) (1971).Google Scholar
  2. [2]
    Cloud, R.S., Ward, J.R., and Jones, R.S.,Effect of induced tolerance and neonatal thymectomy on adjuvant arthritis, Arthritis Rheum.9, 851 (abstract) (1966).Google Scholar
  3. [3]
    Gery, I., and Waksman, B.H.,Competition of antigens in adjuvant disease of rats, Arthritis Rheum.10, 240 (1967).PubMedGoogle Scholar
  4. [4]
    Han, T., and Pauly, J.,Simplified whole blood method for evaluation in vitro lymphocyte reactivity of laboratory animals, Clin. exp. Immun.11, 137 (1972).PubMedGoogle Scholar
  5. [5]
    Kourounakis, L., and Kapusta, M.A.,Effect of Freund's adjuvant on the mitogenic responses of rat lymphocytes, Ann. rheum. Dis.33, 185 (1974).PubMedGoogle Scholar
  6. [6]
    Lay, W.H., and Nussenzweig, V.Receptors for complement on leukocytes, J. exp. Med.128, 991 (1968).PubMedGoogle Scholar
  7. [7]
    Delbarre, F., and Le Go, A.,Hyperbasophilic immunoblasts in the circulating blood in chronic inflammatory rheumatic and collagen diseases, in: The Immunol. basis of connectiv. tissue disorders, 1 vol. (N. Holland Pub. Co. Ed.), p. 219.Google Scholar
  8. [8]
    Newlin, C.M., and Gasser, D.L.,Genetic control of the in vitro responses of rat peripheral blood lymphocytes to phytohemagglutinin and Concanvalin A, J. Immun.110, 662 (1973).Google Scholar
  9. [9]
    Perlik, F., and Zidek, Z.,Breeding experiments on the frequency of adjuvant arthritis in the rat, Ann. rheum. Dis.32, 72 (1973).PubMedGoogle Scholar
  10. [10]
    Rodey, G.E., and Good, R.A.,Modification of the in vitro response to phytohemagglutinin of mouse spleen cells to amyloidogenic agents, Proc. Soc. exp. Biol. Med. N.Y.131, 457 (1969).Google Scholar
  11. [11]
    Rosenthale, M.E.,A comparative study of the Lewis and Sprague Dawley rat in adjuvant arthritis, Arch. Int. Pharmacodyn.188, 14 (1970).PubMedGoogle Scholar
  12. [12]
    Scott, M.T.,Biological effects of the adjuvant Corynebacterium parvum I. Inhibition of PHA, mixed lymphocyte and GVH reactivity, Cell Immun.5, 459 (1972).CrossRefGoogle Scholar
  13. [13]
    Scott, M.T.,Biological effects of the adjuvant Corynebacterium parvum II. Evidence for macrophage T-cell interaction, Cell Immun.5, 469 (1972).CrossRefGoogle Scholar
  14. [14]
    Stobo, J.D., and Paul, W.E.,Functional heterogeneity of murine lymphoid cells. III. Differential responsiveness of T cells to phytohemagglutinin and Concanvalin A as a prove for T cells subsets, J. Immun.110, 362 (1973).PubMedGoogle Scholar
  15. [15]
    Swigle, K.F., Jacques, L.W., and Kvam, D.C.,Difference in the severity of adjuvant arthritis in four strains of rats, Proc. Soc. exp. Biol. Med. N.Y.132, 608 (1969).Google Scholar
  16. [16]
    Williams, R.M., Moore, M.J., and Benacerraf, B.C.,Genetic control of thymus derived cell function III. DNA synthetic response of rat lymph node cells stimulated in cultured with Concanavalin A and Phytothemagglutinin, J. Immun.111, 1571 (1973).PubMedGoogle Scholar
  17. [17]
    Williams, R.M., Moore, M.J., and Benacerraf, B.C.,Genetic control of thymus derived cell function. IV. Mitogen responsiveness and mixed lymphocyte reactivity of thymus cells and lymph node cells from Lewis and Brown Norway rats, J. Immun.111, 1579 (1973).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1976

Authors and Affiliations

  • A. Kahan
    • 1
    • 2
  • F. Perlik
    • 1
    • 2
  • A. Le Go
    • 1
    • 2
  • F. Delbarre
    • 1
    • 2
  • J. P. Giroud
    • 3
  1. 1.Institut de Rhumatologie-Centre de Recherche sur les Maladies Ostéo-Articulaires Unité 5 INSERM, ERA 337 CNRS-Directeur: Professeur F. DelbarreHôpital CochinParis
  2. 2.Institut de Pharmacologie, Faculté de Médecine, LA 206 CNRS-Directeur: Professeur LechatParis
  3. 3.Départment de PharmacologieFaculté de Médecine Cochin, Port-RoyalParis

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