Experientia

, Volume 37, Issue 9, pp 940–941 | Cite as

Interaction of mammalian hemoglobins with dehydroascorbic acid

  • S. Deb
  • S. Som
  • S. Basu
  • I. B. Chatterjee
Article

Summary

Reactive sulfhydryl groups of major hemoglobins from guinea-pig, rat and cat reduced dehydroascorbic acid to ascorbic acid leading to formation of intrachain disulfide bonds. Hybridization experiments indicated that the reduction was carried out by thea chain of cat hemoglobin.

Keywords

Ascorbic Acid Disulfide Disulfide Bond Sulfhydryl Sulfhydryl Group 

References

  1. 2.
    S. Basu, S. Som, S. Deb, D. Mukherjee and I. B. Chatterjee, Biochem. biophys. Res. Commun.90, 1335 (1979).PubMedGoogle Scholar
  2. 3.
    T. H. J. Huisman, E. A. Martis and A. Dozy, J. Lab. clin. Med.52, 312 (1958).PubMedGoogle Scholar
  3. 4.
    C. G. Chua, A. G. Carrell and J. L. Howard, Biochem. J.149, 259 (1975).PubMedGoogle Scholar
  4. 5.
    F. Taketa, A. G. Mauk and J. L. Lessard, J. biol. Chem.246, 4471 (1971).PubMedGoogle Scholar
  5. 6.
    J. Kruh, Methods in Enzymology, vol.XII, part B, p. 728. Academic Press, New York 1968.Google Scholar
  6. 7.
    R. Richterich, Clinical Chemistry, p. 333. Academic Press, New York 1969.Google Scholar
  7. 8.
    P. D. Boyer, J. Am. chem. Soc.76, 4331 (1954).CrossRefGoogle Scholar
  8. 9.
    W. Birchmeier, P. E. Tuchschmid and K. H. Winterhalter, Biochemistry12, 3667 (1973).CrossRefPubMedGoogle Scholar
  9. 10.
    K. Weber and M. Osborn, J. biol. Chem.244, 4406 (1969).PubMedGoogle Scholar
  10. 11.
    A. Riggs, J. biol. Chem.238, 2983 (1963).PubMedGoogle Scholar
  11. 12.
    C. G. Chua and R. W. Carrell, Biochim. biophys. Acta365, 328 (1974).PubMedGoogle Scholar
  12. 13.
    L. M. Garrick, R. L. Sloan, T. W. Ryan, T. T. Klonowsky and M. D. Garrick, Biochem. J.173, 321 (1978).PubMedGoogle Scholar
  13. 14.
    F. Taketa, M. R. Smits, F. J. DiBona and J. L. Lessard, Biochemistry6, 3809 (1967).CrossRefPubMedGoogle Scholar
  14. 15.
    A. Rossi Fanelli, E. Antonini and A. Caputo, in: Adv. Protein Chemistry, vol. 19, p. 73. Academic Press, New York 1964.Google Scholar
  15. 16.
    K. Okonjo, A. Taino, M. Balogun and O. B. Ekisola, Biochim. biophys. Acta576, 30 (1979).PubMedGoogle Scholar

Copyright information

© Birkhüuser Verlag 1981

Authors and Affiliations

  • S. Deb
    • 1
  • S. Som
    • 1
  • S. Basu
    • 1
  • I. B. Chatterjee
    • 1
  1. 1.Department of BiochemistryUniversity College of ScienceCalcutta(India)

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