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, Volume 13, Issue 4, pp 283–287 | Cite as

Insulin-induced factors derived from lymph node cells influence anaphylactoid reaction in the rat

  • M. Koltai
  • G. Blazsó
  • E. Minker
Histamins and Kinins


The effect of supernatants of isolated lymph node cells exposed to insulin has been studied on the anaphylactoid reaction induced by dextran in the rat paw.

Native supernatants derived from rat lymphocytes were shown to increase dextran response only from April to October, while in the intermediate period no potentiation was obtained. The supernatants were filtered through sephadex G-25, G-50, and G-100 gels. Pro-inflammatory activity was recovered in the void volume of sephadex G-25 or G-50 columns, while it ran with bovine serum albumin marker suggesting an approximate mol. wt. of 70-kilodalton. Potentiation was detectable in adrenalectomized rats from November through April.

A fraction of the eluate recovered from sephadex G-25 gels in the range of 1- to 6-kilodalton was found to suppress dextran edema.

Supernatants of calf lymph node cells with insulin were fractionated on DEAE-sephadex A-50 and yielded two distinct fractions, one with pro-inflammatory and the other with anti-inflammatory effect.

These results suggest that insulin affects anaphylactoid reactionvia the release of some lymphocyte mediators.


Albumin Bovine Serum Bovine Serum Albumin Serum Albumin Dextran 
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  1. [1]
    J. Larner,Insulin and glycogen synthase, Diabetes21, Suppl. 2, 428–438 (1972).PubMedGoogle Scholar
  2. [2]
    J. Larner, K. Cheng, C. Schwartz, D. Dubler, S. Creacy, K. Kikuchi, S. Tamura, G. Galasko, C. Pullin andM. Katz,Chemical mechanism of insulin action via proteolytic formation of mediator peptides, Mol. Cell. Biochem.40, 155–161 (1981).PubMedGoogle Scholar
  3. [3]
    M. Koltai, A. Ottlecz, E. Minker andG. Blazsó,Sensitization by insulin of the anaphylactoid inflammation in rats. Int. Archs Allergy appl. Immun.46, 261–274 (1974).Google Scholar
  4. [4]
    M. Koltai, G. Blazsó, E. Minker, J. Lonovics andA. Ottlecz,Anaphylactoid-inflammation-promoting factor: an insulin-induced factor derived from non-sensitized lymphocytes increase anaphylactoid inflammation in rats. Int. Archs Allergy appl. Immun.49, 358–370 (1975).Google Scholar
  5. [5]
    G.B. West,A pharmacological approach to allergy, Fortschr. Arzneimitt-Forsch.3, 409–449 (1961).Google Scholar
  6. [6]
    H. Selye,Anaphylactoid Edema. Green, St. Louis 1968.Google Scholar
  7. [7]
    E.S.K. Assem andA.W. Richter,Comparison of in vivo and in vitro inhibition of the anaphylactic mechanism by β-adrenergic stimulants and disodium chromoglycate, Immunology21, 729–739 (1971).PubMedGoogle Scholar
  8. [8]
    J.H. Baxter,Histamine release from rat mast cells by dextran: effect of adrenergic agents, theophylline and other drugs. Proc. Soc. exp. Biol. Med.141, 576–581 (1972).PubMedGoogle Scholar
  9. [9]
    R.E. Anderson, T.H.P. Hanahoe andG.B. West,Insulin and the dextran reaction in the rat, Int. Archs Allergy appl. Immun.56, 301–306 (1978).Google Scholar
  10. [10]
    G. Blazsó, M. Koltai, A. Ottlecz andE. Minker,Dextran anaphylactoid reaction in Sprague-Dawley CFY rats. Acta Physiol. hung.54, 281–286 (1979).Google Scholar
  11. [11]
    L.T. Yam, C.Y. Li andW.H. Crosby,Cytochemical identification of monocytes and granulocytes, Am. J. clin. Path.55, 283–290 (1971).PubMedGoogle Scholar
  12. [12]
    O.H. Lowry, N.J. Rosebbrough, A.L. Farr andR.J. Randall,Protein measurement with the folin phenol reagent. J. biol. Chem.193, 265–275 (1951).PubMedGoogle Scholar
  13. [13]
    M. Koltai, G. Blazsó, A. Ottlecz andE. Minker,Some characteristics of the insulin-induced potentiation to anaphylactoid reaction in Sprague-Dawley rats, Acta physiol. hung.54, 287–293 (1979).Google Scholar
  14. [14]
    J. Garcia Leme andD.L. Wilhelm,The effect of adrenalectomy and corticosterone on vascular permeability responses in the skin of the rat. Br. J. exp. Path.56, 402–407 (1975).Google Scholar
  15. [15]
    L. Jarett andJ.R. Seals,Pyruvate dehydrogenase activation in adipocyte mitochondria by an insulin-generated mediator from muscle.Science 206, 1407–1408 (1979).PubMedGoogle Scholar
  16. [16]
    J. Larner, G. Galasko, K. Cheng, A.A. Depaoli-Roach, L. Huang, P. Daggy andJ. Kellogg,Generation by insulin of a chemical mediator that controls protein phosphorylation and dephosphorylation, Science206, 1408–1410 (1979).PubMedGoogle Scholar
  17. [17]
    K. Cheng, G. Galasko, L. Huang, J. Kellogg andJ. Larner,Studies on the insulin mediator. II. Separation of two antagonistic biologically active materials from fraction II, Diabetes29, 659–661. (1980).PubMedGoogle Scholar
  18. [18]
    L. Jarett, F.L. Kiechle, D.A. Popp, N. Kotagal andJ.R. Gavin III,Differences in the effect of insulin on the generation by adipocytes and IM-9 lymphocytes of a chemical mediator which stimulates the action of insulin on pyruvate dehydrogenase. Biochem. biophys. Res. Commun.96, 735–741 (1980).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1983

Authors and Affiliations

  • M. Koltai
    • 1
  • G. Blazsó
    • 1
  • E. Minker
    • 1
  1. 1.Departments of Pharmacology and PharmacodynamicsUniversity Medical School of SzegedSzegedHungary

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